FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030327
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC668140.2669321898990772TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT425280.1699058905622767No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC319430.1276171901389862No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC318390.12720169416883767No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG304170.12152058580776831No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT303570.12128087659422108No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA273670.10933536745245079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA163950.051.64082316
ACGTCTG165350.051.2880515
ACGATAT164150.050.91570335
ATCGGAA169100.050.2381822
CACGATA168400.049.88011634
CGATATC161750.049.31265336
GATCGGA173200.048.9069861
CACGTCT174150.048.81689514
TCGTATG108700.048.6834643
TATGCCG107700.048.6804346
CGTATGC111100.047.60028544
TCGGAAG179550.047.4901923
TATCTCG135550.047.12186439
CTCGTAT123250.045.03761742
CACACGT193000.044.3036212
AGTCACC194100.043.04317528
GTATGCC130200.041.82702345
GTCTGAA205550.041.2746117
CAGTCAC209450.040.20616527
GCACACG218400.039.32753411
Produced by FastQC (version 0.11.5)