FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030327
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC668140.2669321898990772TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT425280.1699058905622767No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC319430.1276171901389862No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC318390.12720169416883767No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG304170.12152058580776831No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT303570.12128087659422108No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA273670.10933536745245079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA164100.051.72160716
ACGTCTG165900.051.26571315
ACGATAT164300.050.9331435
ATCGGAA169300.050.2614562
CACGATA168400.049.9632734
CGATATC161000.049.5858636
TCGTATG108400.048.88278243
TATGCCG107650.048.80059446
GATCGGA174050.048.7887271
CACGTCT174650.048.75731714
TCGGAAG179650.047.5611083
CGTATGC111150.047.547444
TATCTCG135900.047.0777939
CTCGTAT123150.045.15946642
CACACGT192500.044.4732612
AGTCACC194900.042.97417428
GTATGCC131000.041.6250345
GTCTGAA205500.041.4038917
CAGTCAC209800.040.25580227
GCACACG218400.039.359611
Produced by FastQC (version 0.11.5)