FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG45.0TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC22.5TruSeq Adapter, Index 9 (100% over 50bp)
GTTACCAAGTACACTAGTCTCAATGGCGTGGAGCGCCATTCCACGTCTAA11.25No Hit
TGGAGGGCTACTGTCCAAATTAGACAAAAAGTGCCTCACAAGAGAACATT11.25No Hit
GTCGGCGATGCAGAAGTCCTGGTACACGGCGAGGGAGGAGAATGGCAGCA11.25No Hit
GTTTCAGACAAGTGATCCCTATTTCGCTCGCTTTCGTCTCGGCTACTTGC11.25No Hit
GGCCTTCAAGTTAGGACATAATGCCTTCGGTCAGTGATAATGCGGCAAGC11.25No Hit
GTGGTGGTGAATAAATGTTGTTGCAAACTTGATTACTCGCAGCTATGCTA11.25No Hit
CCAGCATGGACGCCGGGTTAAGCAGCATCCTGGAATGGAACGACCGAGAG11.25No Hit
GCGGTTAGATAGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT11.25TruSeq Adapter, Index 9 (97% over 43bp)
GCCAGAATACCCAAACGACAACCACGAAATTGCGACGATATAAAATAAAC11.25No Hit
GGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTG11.25No Hit
CCCATCTTATCCGATTCGCTGGCATGGGCCTCGCCTTTCTTGCGTTCCAG11.25No Hit
GGGCAAGCTACTTTCAACGGTATCATTCGAGCGCTAAGAAAAAAAAAAAA11.25No Hit
AAAAAAAAAAAAAAAAAAGTTGCAGTATCATAATAGGAAAACCGGGTTTC11.25No Hit
TGACTTAAGACAATTGCAAAATCTAGACATATCAAGATCGATCTACAGAT11.25No Hit
GTCCGACGTGTACGCGTCCCACGGCCGGAAGCTGGTCCACTTCCACTCCG11.25No Hit
CACCCAAATGACTCCAGCCCCAAAGATCTAGATCGGAAGAGCACACGTCT11.25Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGGAGCTGCACGCGCACGTGAAAATCGCTCGGCAGAGAGGACGGACA11.25No Hit
GTCACCGCCGTGGTGTTGCCGTGGCTGTACTGGAAGTGGAGCTGGCCCTG11.25No Hit
AGGACAGAGGACTCGTCATTTCTGTAAGATATGCTCTACTGGGTTCACAA11.25No Hit
CCAGACCAGGAGCGGCGTGGCGGCCGGCGGGGTCAGCGGGTGGGTGGCCT11.25No Hit
CCCCGTCCACCAGCCGGCCGCCCTCGGAGGAGGGAGAAGAGCCGCCGTCG11.25No Hit
TATGTTATGTCTAAGAAAACGTAGAATGGGTTTATAATTGTTGTGAAAAC11.25No Hit
GCACGTCACGACGAGAGCCACCAGCAGATATATGAGCGCCGGCGAGTCCG11.25No Hit
GCTTCCACCACCAAAGAATGACTGGAATATGTCAAAGGGATCGTGCATCC11.25No Hit
AATACGCCGGATATCTTAGCTTGTAGGAGCACAAAGCCACTTGAAGATAA11.25No Hit
TGCTCTTCCGATCTGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAG11.25Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGACAGAGCGGCCGGCGACATTTATTTATTTATTTATTTCTTTTCTCT11.25No Hit
GTCAAAGTAATATGTTGGGAATTTATTCCCGTATATAGACCATCTAAGAG11.25No Hit
CACAGCCATCCAAGCCTCTGATCCATGTCCATGCCGCTCAACTTTGCCTT11.25No Hit
GGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA11.25TruSeq Adapter, Index 9 (100% over 47bp)
CGGCGGCCGACCCCAAAGGGCACGAATCGGAAGTCGTTGCCGTGGGCCTC11.25No Hit
AAAATTTCCGGAATCAAGTGATACCGATAAGGAGAAGCAAAAGACCTATA11.25No Hit
CTAGAGACAGGCCTTTTACCCTGGTTGATGAACCATGACCGTGCGGCTAG11.25No Hit
ACAGCTCATGATGCTGCCCAAAGTCAAAGCCAATCGCCCGGACCGCGACA11.25No Hit
CATTCTCACAATTCGATGAGTTTCCTCCCAATAATCAACTATTCAAAATT11.25No Hit
CTGCCTTCGCCTCCTTAATGGCTGCGCGGATGTCATCATATCCGGTGTTC11.25No Hit
TTCAGGGTTAACTCCTGTTGATCGATGACTGCTTGCTGATACCAAGTGAT11.25No Hit
TGCAGTTGTAGTCCCATCCTTTTTAAAAACTGGTTTCAGTTTCGCCAATC11.25No Hit
TTTGGACCGTTAATTGTAGGTATATATATCAGCAATGCACTATTGTATCT11.25No Hit
GAAAAAAGCAATCTCGCGAGTGGATTGCATCGGATTTTATGAATGTGTGT11.25No Hit
TGTGTAATGAATTGGAGAACAACCGCAGCGAGCGGAGCAGCCTGCAATTC11.25No Hit
GTGGAAGAAAGAAACCATCGTTTTTGCAGATGAGATTCTTTTTCCAATGC11.25No Hit
GGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT11.25No Hit
CCTCAATTGCAGCTGCTGCTCCTCGCCCGGACGCCTTCTCCCTACTTGCA11.25No Hit
GTCTGTTTCTCAGTAGCTCAATATGGCATAAATCAATAGCTCAATATGGC11.25No Hit
GAAGCTCGGATCGGACGTGATGGCCTTCGCGAGCGTGTCCGTGACGGCGG11.25No Hit
CTGGCCATCAGGGTGGGGCAGGCTCAGCTCGACGGCCAGGGGCTTCAGCG11.25No Hit
CCCAGGGGCAGCCACGCGAACATGGCGGCCGTGCCGGCGCCCGTCGGCCG11.25No Hit
AACACAGTGTCCATGCCCCACACCGTGTGACGATCAAAGTGGCAATGCAT11.25No Hit
TCCTACTATAAAAGCCTATACTCAAAAAAAAAAAAAAAAATGGAAAATAT11.25No Hit
CAAACTTTACCAGTAGATTCCACGCCCTTGGAGACGTCGCCTTTCTCGTG11.25No Hit
CGACGGCGCCGCGACTTGCCTCGAACTGTAGGTCAGGAAGACAGCGAGAG11.25No Hit
ATACTGTGGACGTCCTCCTCTGACACCCCTTCCTTGTTGTCGTCTGTGGC11.25No Hit
GTTAGGCGTACTCATGAATGTGAACTCGACTTTGTCTGGATTTTATGCTT11.25No Hit
CTGGTCCGGAAACACCGCTCCGACGATCCTGGGACTGTTCCGCAGGTACT11.25No Hit
GTAAAAAAAAAAATCTAATCTAGGAAGCTCCTTTGCAGTAAGTACGTGTA11.25No Hit
CTAGCCTTTGTGCTGTAGTCAGTTGCGCAGGTGTTCCCTCAGGATCTTCA11.25No Hit
AACAAAAAAAAAAAAAAAAAAAAGACAGAACTCGGGGGGGGGGGGGGGGG11.25No Hit
TTTCCCATGGTGTTTCTGACGTATGAAACGGACACTAATGTCTAGGTAGT11.25No Hit
CGATTTAACCACCAGTATGACCAGAATAACCCGATACGACCACTTTATTG11.25No Hit
CCGCCGCCCCTCTTCGACGCCGCCACGGCCGCGGCCGCTTCTCCCATCCC11.25No Hit
CGGATGGGCTGAACAACAGGCGCCACCGGGAGCCCTCCGAGCACCTGGGA11.25No Hit
AGCTCGATCACGGTGGCGTGCGCGCCCACCGCCGCCAGCAGCCGCCTCAT11.25No Hit
CCCGTATCAGAAGCTTCCAACTGATCATTTGAAAGTCTGGAATCGAGGAT11.25No Hit
CTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTC11.25No Hit
CCCGAGTCATGATGGAGTACACGAAGCTGAACGGAGGGTAGCTCGCCGGG11.25No Hit
ACTACTGTCACTGTACTGGAGCTTGTAGAAAAGGCTCTGCAGCGACAAGT11.25No Hit
GCTCGTCGAAGTACATGCTCCATATGGCGTCGTTAACGTTGACTGACGCG11.25No Hit
GTGAAATTCGTTCTCTTACATCTACATTAACATTGGGGAGGTAGCTTTAA11.25No Hit
TGGGGCCTCACAATCGGCACGTAGCCGAGCTCCTCTCTAGCTTTCAAGAA11.25No Hit
TTTGGTTTGTGGTGGGGGTGATATAATGATAATGGAAAGCATGTATGATA11.25No Hit
TTCCAATAATATCCTCGGAATCTGCCCATGGTTTTATTGAAAAAGGCACC11.25No Hit
CTCTGGAACTCTGCTGGCTGTGTAGCTTCCAGCACCTCCTCTAGCTCACC11.25No Hit
TGGGGCCTCACAATGGGCACGTAGCCGAGCTCCTCTCTAGCTTTCAAGAA11.25No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTA50.070.050
AGCCTAT50.070.013
CCTACTA50.070.02
TAAATTT50.070.063
TATACTC50.070.017
TAAATGG50.070.055
CTATACT50.070.016
CCTATAC50.070.015
CTATAAA50.070.06
AAATTTC50.070.064
TCCTACT50.070.01
AATTTCT50.070.065
GCCTATA50.070.014
TTTCTAA50.070.067
TAAAAGC50.070.09
GAAAATA50.070.043
AAATATG50.070.045
ATGGTTT50.070.049
TATAAAA50.070.07
GATAAAT50.070.061