FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26621778
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC895760.33647639913457317TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG141400.052.2761543
CGTCTGA165750.051.01601416
CGTATGC144400.049.9054844
CTCGTAT141650.048.7491642
ATCGGAA175550.048.4330752
GATCGGA179850.047.0212861
TATGCCG154150.046.1813346
AATCTCG156900.046.1300839
ACGTCTG184350.046.1156515
TCGGAAG187250.045.5565383
GTATGCC162150.044.67992845
CACGTCT192400.044.13168714
ACTAATC169050.044.0779336
CTAATCT173300.042.2900337
CACACGT202950.041.9246712
AACTAAT207150.040.4313735
GTCTGAA212800.039.99947417
CAACTAA210550.039.96132734
TAATCTC183550.039.9094338
CCAACTA210350.039.88285433
Produced by FastQC (version 0.11.5)