FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27265418
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC771510.28296283592644716TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG126150.049.0238943
CGTCTGA145050.047.89639716
CGTATGC135800.045.9783744
ATCGGAA152400.045.729592
CTCGTAT129700.044.9564442
GATCGGA155100.044.571781
TATGCCG143750.043.6546946
TCGGAAG166250.042.1098183
ACGTCTG166250.041.9992315
GTATGCC153600.040.99193645
CACGTCT173600.040.20102314
CACACGT186150.037.52942712
AGTCACC183900.037.49437328
GTCTGAA191950.036.48539417
CAGTCAC189600.036.23794627
TCTCGTA168100.034.8325441
GCACACG206950.033.7913911
CGGAAGA213650.033.2591064
GCCGTCT187650.030.83065649
AGCACAC229150.030.77751510
Produced by FastQC (version 0.11.5)