FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26746497
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT499420.1867235174759521TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA130800.044.09704216
ATCGGAA137300.042.2958342
GATCGGA140300.041.241191
TCGTATG86200.040.683445
ACGTCTG146900.039.35945515
TCGGAAG149600.039.0528683
TATCTCG108850.038.7127541
CTCGTAT97550.038.17430544
CGTATGC93900.037.75727546
CACGTCT157650.036.7643114
AGTCACA160250.036.060328
TATGCCG103350.035.55785848
CACACGT168200.034.64645812
GTCACAT166900.034.56052829
CAGTCAC172750.033.53206327
ACATTCC172800.033.39914332
GTATGCC112600.033.32062547
GTCTGAA175450.033.1940317
CCTTTAT184750.030.74627137
GCACACG190750.030.67907711
Produced by FastQC (version 0.11.5)