FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGAAAGACACCCTTAATTCTTATGTACATTAATGCCCTATTGCACTA11.1235955056179776No Hit
TGCACAGTTATTCACAGCCACATACAAACGAGCACATCTGATATATTTCA11.1235955056179776No Hit
TGTAAATGTGGTATTCATTTGTTAAACGGTCAACTTGTTCAGGTGTCATC11.1235955056179776No Hit
TATAAATAACAAACTCAAAGGATTCTGGGAATTATATAACAGGCTAGCCT11.1235955056179776No Hit
CGGCTGTGCATAATGTCATCAACTGATTGGGAGTCATACACTGAGAAATG11.1235955056179776No Hit
GTCTTGAAGTCCTGCGCGATCTCGCGCACCAGGCGCTGGAAGGGCAGCTT11.1235955056179776No Hit
CGCGTCCAGTCCAGCAGCTCCTGCCGGCACTGGTCCTGGTTGGGCGCCGG11.1235955056179776No Hit
CATCTTGAAAAAAACAAAAATAAATTAAATAAATATAATTTAAAAATTTA11.1235955056179776No Hit
CAGGAGAGCAAAGGGCATCAATCAAACCACGTTCCTTCCCTTCCTTTGCC11.1235955056179776No Hit
CTTATCATAATAAGCATTATGAGCACATCTCAACACCATATGTTCGTAAT11.1235955056179776No Hit
ATTTTAGGTATAGGTAAAGTAACAATTAAAAAATTAAAAATAAAAGATGG11.1235955056179776No Hit
GTGCTCTTGCAGATGGGGCACACGTTCTTCACCACCAGCCATTGCTTGAT11.1235955056179776No Hit
CCCCTTCACTGTATCCTTGATCATGTAATTCTGGATCCTCCACCATCTTT11.1235955056179776No Hit
GGGGTGCCGTCCGGCCGTCGGTACGGCTCCGTGCGGACGGCGGCGACCCG11.1235955056179776No Hit
CTCCCATTCACCATCCTCCTCATCGTCCCAGTCCTCAGGCTTGGCTGCCT11.1235955056179776No Hit
CCAGCTTCTTCCTCATGGTGCTGACGGCAAGGCGCTGGAAGCAGCGCCGG11.1235955056179776No Hit
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC11.1235955056179776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACCAAAAGATCGCGTATG11.1235955056179776Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGGATATTAACAAAGACAGCCCAAGATGAGCCACTGGAAGGATGTGACTG11.1235955056179776No Hit
AAAAAAAAAACAAAAAACATAAGCAAAAACAAACACTAAAGACTCAAAGA11.1235955056179776No Hit
GCACTTTTGCGGCCATCTGTTGCCATGTCTCCTTAATTGAGAAGCTCAAC11.1235955056179776No Hit
AACAAAGACATAGCATACCTTGTTGTTCACCGGTCTTCCTATCCTTAATT11.1235955056179776No Hit
CTTGGTGAAAAGGTAGGCGGCTAGTGGCTTAGGCTTGGCATTGATCAAGT11.1235955056179776No Hit
GGATGGGGCCATGTTCATGCTGTCGGAGGAGGTCCCACAGGAGACTCCGC11.1235955056179776No Hit
GGGGTTCCACTTCTCCTTCCAGTAAAAGGTCTCGGCAATCTCCGACCAGA11.1235955056179776No Hit
GCAACACACAGTACGTGAACCATGCACCGGCCGGCGGCCGGCCGGCGGCG11.1235955056179776No Hit
GCCTGCTTGCCGCCCTTGGATTCAGCGATGAGACGGAGGTAATCCCAGCT11.1235955056179776No Hit
GGCTCAGGTTGAGATCGAACAAGAAGTTGAGGTCACCCAGCTCAGGTGGA11.1235955056179776No Hit
CCCTGACTTGGCATGCTGTCAGGTGCGGAGACGAGGCTTCTGCTACACAC11.1235955056179776No Hit
CTTGTTGAACACATGCAGCCACTTGAGGTTCTCCTTGTCCTCATCGTCCA11.1235955056179776No Hit
TTGCGGTAGAAATACATGGTAAACGTGGTGCGTGTTCTTCTCTACGCGTG11.1235955056179776No Hit
CCCTGGTTATAACGGTTGCAGCAGCAGGTTGCAATTGGGCAGTATGAACC11.1235955056179776No Hit
CTCGCGGACCGGCTTGCCGCCGCCGCCTGCAGAGCTGCCCTCGTTCTCGC11.1235955056179776No Hit
TTGCGAACAAGTATAACGGGAATGCGAGCAAGGGGAACGGCATGGTGAAC11.1235955056179776No Hit
TAAAAAAAATAAAGATGCTATCAGAGATTTGCTGGTTGAAGTAGGCATTT11.1235955056179776No Hit
CATCTACAGATGAAGGTATTGTCTTGCTTCTCCTATCTTTTTTCTTCTTT11.1235955056179776No Hit
ACGCAGATAATATTGCCCCTTTTCTCTTTCTCTTTTCTCTCCCGGAAGAC11.1235955056179776No Hit
GCTGGTCACCGACCAGACCGCCACCACACCCAGCCAGCCACGGCATTGGC11.1235955056179776No Hit
ATTCGACCTCACAACCCAAATGAATGGTTTGCCAGAACTGTACAGCCCGT11.1235955056179776No Hit
CTTGATGTCATGTGTATGATTGAGTATAAGAACTTAAACCGCAACGCAAT11.1235955056179776No Hit
CCGTGGATGTCGCCGCAGACAGTGACGGGGCAGCGCACGGGCTGCACGTT11.1235955056179776No Hit
ACACGAAGGACGCGACCTCAGTGTGGTCCAAGAACGCGCCAGTGACGCCT11.1235955056179776No Hit
CCTTTGTAGTGCATATTGTTTTTTCATTGACAGAGAAAGTACAAACATCC11.1235955056179776No Hit
CTTCGAGGTCAAGGGAGCTTCTAATGGATGTTGACGACTTAGATCTTGAC11.1235955056179776No Hit
ATATATGAGGATCCAGTTGCCGTCGAGGAGATCCGGCGACTCGACGGGAG11.1235955056179776No Hit
GTGCAGCGTCCTCTGCGCGGTGGCCCACTGCGCCTCCCTCTCTTCCCGCC11.1235955056179776No Hit
GACGAAACTACAACATGCTACAATGCAGCGAAAGAGGCCAAGTTTGGTTT11.1235955056179776No Hit
CCGGTGTAGGGGAGCAGCGACACGTCCGGCGCGTGCCAGGCGCGGCGCCT11.1235955056179776No Hit
GGTGCCGCAGCCGCTGCGGCCGTTGCTGGAAAGTGTGTGCCGCCTGCTCT11.1235955056179776No Hit
CTTGACGACATTGATTTATCATGCCAATAACATCAGGGATCCAGGATCCG11.1235955056179776No Hit
TGTAATTCTTCAATTGGAGGTTTGTAGGTCAAGTATAGCTCAACTGCACG11.1235955056179776No Hit
CGCAATAGCAAGTACTAGAGTTACGTTGAGGTGCAACCCCGCATATTTAT11.1235955056179776No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAAGCCAATATCTCGTATG11.1235955056179776TruSeq Adapter, Index 6 (97% over 49bp)
CACTTTATTATACTTAATTGTATTCCTTCATCGCATAGCTTCCTAACTTC11.1235955056179776No Hit
AAAAAAGTCGGTTTTTTACATTACATACAGGAACCAAGCGTCGTCATCTC11.1235955056179776No Hit
GTGTGCACAGGCTTCAAGCAAAGTTTCAGTGTCCAATGGCATGCTAGTGG11.1235955056179776No Hit
CCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCG11.1235955056179776No Hit
CTCGGGGAAGGACAGCTTCAAGTAGTCGGGGATGTCGGCGGGGTGCTTCA11.1235955056179776No Hit
TAAGAATCCTCATAAGACTATTCCCCCTTTTCCCCCATTCAGATGCACGA11.1235955056179776No Hit
ATAGTACGTACGTATAAATGTATAATAATATACCACTCGATCTTCTTATT11.1235955056179776No Hit
CGATGACGAAGATGAGCTCGGTGCCTCCCGGGTGGGAGTGCAGCGGCGCG11.1235955056179776No Hit
CATGGTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGC11.1235955056179776No Hit
CGCATTTTTTACAATTCATCATTCAAACATGATAGCTGTTGACAACAATA11.1235955056179776No Hit
TCTCGATCTCCGCGCCGGAGCGGCGCGCGGCGTTGACGGCCTTCTCATCC11.1235955056179776No Hit
CAGGCATCTCTCCTCTCTTGCTTGCTCGAGCCCCTAGATCCATACGCTGA11.1235955056179776No Hit
GGGGCCTATCCTTTGGATAGTTGGCAAGGGAGCAAGCATCAAGAAAGCTT11.1235955056179776No Hit
CATCTTTGGGACCACTGTCGGCAGGGGCATCTTGAACGATAGCCTTTCCT11.1235955056179776No Hit
TGAGTAAACTAGGTAAGAAGCAAGAACCTGAGCAACACTGTTTGCCATGG11.1235955056179776No Hit
CTGGTTTGCAAATTGCAAGGAAAAAAATGAGTAGCCATCTGTACATTTTG11.1235955056179776No Hit
CTGAAAAGGGAGCTGGTCTATATTGTTTCTTGCACCAGTCCATCTAAGAC11.1235955056179776No Hit
TCTGCACTTTTGTTTTTGCTGGCAAACCCAATTTTTCTACCGTCCAGTAA11.1235955056179776No Hit
GGGAAGTACTTGTCGATTAGGGACATGTAGTAGGGCTTCACCTCCACAAC11.1235955056179776No Hit
TTTTTTTCTAAAACACATTCATATAACAATATCTGTAGAGGATAATAAGT11.1235955056179776No Hit
GTGGATCCAAAGAAGGTAGCAGTGATTCTAGACTGGAAAGCACCAAAAGA11.1235955056179776No Hit
ACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCT11.1235955056179776No Hit
CACCTTGACACCCAAGCCCCACACGCTCTCCATGTATTTCGATATGGTCG11.1235955056179776No Hit
CGTCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTG11.1235955056179776No Hit
CGAGAAGCAACTTTGGATGAGCTGCGCAACTGCATCTTTGTCTCTGCCGA11.1235955056179776No Hit
GGGCCCATGCCGGGACGAATTCCTGCACCGACCTTGACGCAGTTCATGCA11.1235955056179776No Hit
GCTGTGGCGTCTCCTGATAAGTCAGCTGTATCTGCACAAGTATCTCCCAA11.1235955056179776No Hit
GGTGTGTCTGAAGACAGCTACGGTGTACTCATATATATGAAATAAATCTT11.1235955056179776No Hit
TGCGCCTTGGAAAATCCGTGCCTTCAGGTGGCTTTCGTAGACATCAGTGG11.1235955056179776No Hit
GTTTGTTATCTATGCTAATCCATTGTAGCACAACACCTGCCCGCCGCGTA11.1235955056179776No Hit
CCTTACGAGGGCTCTCAGGGCTTTAGTTTTTTTTTTTTTTTTTTTTAACC11.1235955056179776No Hit
AAATTTAAGTTGGTAAAAAATAATATCTAAGTCGTGCAAAAAAAAAAACC11.1235955056179776No Hit
GGCCAATGTACCAAGAGGCTTCTTCTCCCAAATGCATCCCGGCCAAACCA11.1235955056179776No Hit
CCATGCCTATGTTCAGCAAGGCGTTTACGTCGTCAGCATGTTCCCAGAAC11.1235955056179776No Hit
CCGTGCTTCAACACGAAGCCACCACAGTAGGACCTTCTTCCCACAGTTGC11.1235955056179776No Hit
TACAGAGAAACCCTGTCTCGAAGAAAAAAAAAAAAAAATCCCAGCGGAGT11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC50.070.060
CGCTGGA50.070.034
CCGTGCT50.070.065
ATCTCGC50.070.019
AGCTTGC50.070.046
GGGCAGC50.070.042
AGCTCCG50.070.061
CAGCAGC50.070.057
TGCGCGA50.070.013
GGCGCTG50.070.032
GCGATCT50.070.016
CCTGCGC50.070.011
GAAGTCC50.070.06
GCTGGAA50.070.035
CTCTTCT50.070.070
TCGCGCA50.070.022
AGGGCAG50.070.041
GGATCAG50.070.053
GCGCGAT50.070.014
AGGCGCT50.070.031