FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23046101
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA126550.050.5011216
ATCGGAA134300.047.748832
GATCGGA139050.045.9158251
TCGGAAG144650.044.4778823
AGTCACA151300.042.1723728
ACGTCTG153600.042.01768515
GTCACAT156700.040.62964629
CACGTCT159050.040.59991514
ATCGGGG84200.040.48614540
GTCTGAA163250.039.44818517
CACACGT165850.038.95722612
CAGTCAC164550.038.6701927
TCACATG178050.036.0329530
CATCGGG118100.035.79947339
GCACACG184900.034.94336311
CGGAAGA187550.034.920194
CACATGA191150.033.65332431
ACATGAG198350.032.5554532
AGCACAC201350.032.3668810
TCTGAAC205400.031.55752618
Produced by FastQC (version 0.11.5)