FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACCCGTTCACCGACACGTCATTGCACTGGCACTCAGGCGGCTGCT10.684931506849315No Hit
GAAGGAAACCAGCAGCACCGCCTGGCGAAGGCAGCGGCGGCGGCGGCGGA10.684931506849315No Hit
GCCCAAGCACCTTGAGAGTGAGATCAGTTATAATATTAGTCTCCTGTCCT10.684931506849315No Hit
TTCTTGGTCTGATTCTTTTGGTTTACTTTTCTATTTTTTACAGATCGTTT10.684931506849315No Hit
TGCTGGATCTATGCTTTCGCTGTGTTTGGGCCGGCACTGGCGGCAGACAC10.684931506849315No Hit
GGCCGGAGGACCACTTGCCGACGACCGGCCCGACCGCGACCGCGCCGGGG10.684931506849315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGGTTGC10.684931506849315TruSeq Adapter, Index 5 (97% over 45bp)
GTCCTCAAGCTGCTTCCCAGCAAAGATGAGCCGCTGCTGGTCTGGAGGGA10.684931506849315No Hit
TCCGTGTGACGTTGGTGTCGGCGCCGCGCCACGGCTTGGGCAGCGATGGG10.684931506849315No Hit
GCGCCGTCGCGTTGAAGCGGTACGTGTAGTTGCCGCCCGGCTTGACCGGA10.684931506849315No Hit
AAAAAAAAAAAAAAAAAAAGGCAGAGTCTCAATTTGATGCCTTGGTTGGC10.684931506849315No Hit
AAAAAAAATAAATTTTCTGTCAGGTTTGGTTTGGGGTGTGGTGATGCATA10.684931506849315No Hit
GCCGCCGAAGGACCCGGACGAGTTCTCCGGCGTCACCACGGGTGTTCCGG10.684931506849315No Hit
ACGGTCTAAAAGTTATGGAGTCATAAAGTTTTAACAAAAAAAAAAAAGGT10.684931506849315No Hit
CCTCTTTGCTGATGCAAGTGTCATGCTCTCTCATGTATAGCATATTGTCA10.684931506849315No Hit
GCAGCTGCATGAGGATCTTAAACTTTGAGGTTGCTTTGTTCGTACCATAT10.684931506849315No Hit
TCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTC10.684931506849315No Hit
GCTGTGTACATGACAGCAGAGACGACCGTCGGGGACATCCAGTACATGAC10.684931506849315No Hit
GTAATATCTGAACAGCCTTTTCATAAACAATGTCCTCTTTACTCTTCCCT10.684931506849315No Hit
CGCGGTCCCGCGTGTTGTAGTACCCGGCCGAGAGCTCCGCCGCATGGGAC10.684931506849315No Hit
CCACCGTGTCGACCACCTCGATGGCCACCTCTGCCGTCTTCTCTATCTTG10.684931506849315No Hit
CCTCCGCATTGCAATGAACGGAACAGCGGCATGGACAGCTGCAAACCACT10.684931506849315No Hit
GTTACTGATCTGCAGTCAAAAACTCAAACACAAGATTTTGCTATTATGAA10.684931506849315No Hit
CCTCGGACAGGAGCAGCGGCACGAGGAGCCCCGCGGCGCCGGCCTCCCCG10.684931506849315No Hit
CACCACCGCCGGGTCCTGGGACGACCGCTCCACCCGCTTCTTCACGGGGC10.684931506849315No Hit
GTACAGGTCGCCTTCCTTGTGGGTGTGGATGACGCAGTCGGACTTTGGGT10.684931506849315No Hit
GCACGTCGTCGGGGCGCGGCGTGAAGCGGCGCTGGAACACGATGGTCCCC10.684931506849315No Hit
TTAAGTTGTTACCATATTCAGCTACTGTGAAGTGTGTTGTGACACACATG10.684931506849315No Hit
CTTTTGATGATTAAAGCCATTTCCTCATTGTCGAGTTTGGAGGCGTCAAT10.684931506849315No Hit
CTGCTGCATATCTCTTCACACCACTCTTCTTTTCTGTGCAACAACCGCTC10.684931506849315No Hit
CTTCGCTTTGGTTCTCCACATCACGTAGTCAATAAACGTACCCTGACTAA10.684931506849315No Hit
GCGGACGTGGACAGCGGCCCCGTCGCGACCCTCGAGGCGCTCGCCGATAA10.684931506849315No Hit
GTGGATTCCCTTCTGCACTGTTAGACGAGTGATGATTCCAACAACAGGGC10.684931506849315No Hit
GCGGCGAAGCCGCGCAGCTGCACGGCCAGCACCTTGCGGTAATTGGGCGG10.684931506849315No Hit
TGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTCCTCGAAGTTCATCA10.684931506849315No Hit
GAGTAAAATAACATGAAGGCATCAACCAAGGCAAGGAGAAGTGGTATCTG10.684931506849315No Hit
AAAAAATAAAAATAAACAGAAATAGTCGTTAACCAAAAAACCGCGAACAT10.684931506849315No Hit
TTTTGTCATCCTCATCATCCAAATGTTCATCTGTATCAAAGTTGGTGTAT10.684931506849315No Hit
CGAAGGACTCGATGTACAAGGCCTCCAGCTCTTGGAAGCTGTTTATGACA10.684931506849315No Hit
TACGGCCCCTAGGCCTGTACCTGTTATCCATACCAAGACCCCATCGCCCA10.684931506849315No Hit
GGCACCAGCTTAGTTACACACATAAAAACTAGAAATAATCCACTTTTTCA10.684931506849315No Hit
GGTGGATGGACGTGAAGAGGAACCTACCACCGAGGGAGGGTACCAAGACT10.684931506849315No Hit
AGCCTTGGTGCCCCGCTTCGTGAACCAGAGGTCCTGTGGCGGGTTGGGAG10.684931506849315No Hit
CTCTTGTTCATCTCGGCAGCCTCCTTCTCAAACCTCTCGATGACACGCTT10.684931506849315No Hit
GCGGTAGGTGACGACGGGGCCATGGCCCATGCCGGGACGAATTCCTGCAC10.684931506849315No Hit
CGTTTCGCATGTCGCGTCGTCGACCACACCCCCGCCGGCGGTGACGACGA10.684931506849315No Hit
CCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGATGTCG10.684931506849315No Hit
TTTAGACCGATTGCTATCCTTGCCAATTGGAGCAATCTTTGGAGCATTTG10.684931506849315No Hit
TAGCGCGCAGTTTGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGC10.684931506849315No Hit
CTCTCAGGATGCCTTTCGAACTTCAGTTCGTAGTAATCCAACCAGTTTTG10.684931506849315No Hit
CTCTATTGAACACATCTTTGCAGAGGCCTCTAAAAAGGGTGTTTTTGGCC10.684931506849315No Hit
ACGGTCTAAAAGTTATGGAGTCATAAAGTTTTAACCAAAAAAAAAAAGGT10.684931506849315No Hit
GTAGGAAACAATCTCAACATCAGTTGGGTGGAAACGAAATCCAGGAGGCA10.684931506849315No Hit
ATTTTTATTTTCATCCATTAAAAAAAAAAAAAAAAACCAAAAAACCAAAA10.684931506849315No Hit
CGGCGCTGCGCCGGCGCGGCCAGCTCCTTCTGGACCTTGTGCGCGAACGC10.684931506849315No Hit
GGAGGTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCA10.684931506849315No Hit
GGCTCCTCCTCCCGGGGCCGCCGCCGCCGCCCCCGCGGCCGCTGCCGCGG10.684931506849315No Hit
GGCTAAATAAAATTCCAACTCTACAGATTTGAACTTTGGATATGAAGATT10.684931506849315No Hit
TCCAGGGCAAAAAGGAAAAACAAACAAAGCAACTCTACGGCGAAGAAATC10.684931506849315No Hit
CCCCGTTCCGTGGGGCTCCAGGTGGTGTAGAACAGGTCGGGCTCCTTGCT10.684931506849315No Hit
AGCATCAGCCACCGGGGGAGACGCGGCGGTGGGGGGCGCCCTCTTTGTTC10.684931506849315No Hit
TTTTCCTGACGACCCATCTTCAGGATCTAAACGGATCACATCCTTCATAT10.684931506849315No Hit
TAAAGAAATAAATTAAAGACTTTTTAGCATTTAATGAAAATGAAGCCACA10.684931506849315No Hit
CACGACGGTGAGCAGCCCGCCGATGCACTGCGCGAAGGCGTCCTGGATAA10.684931506849315No Hit
GGGAGAACACCAACCAAGGTTTTCCACATTCCAGGAGGCTTTAATCCAGG10.684931506849315No Hit
GCTGCCTCCCACACGTTGTTGAGCTGCCCGTACGTGAGAAGCGCGAGGTT10.684931506849315No Hit
GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGA10.684931506849315No Hit
TCTTTTTACTTGTGCTGATTTGTCAAAACAATGCAAAATTAGAGCTTGAA10.684931506849315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGG10.684931506849315TruSeq Adapter, Index 5 (97% over 49bp)
TTGCATTCAAACATTTCGATGATCCAATATAGCACAAAGGTTCCTTTTGA10.684931506849315No Hit
CCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGGT10.684931506849315No Hit
TAGGGGCACGATGGGTAATAAAAAAAATTCGATAATATAACAGTAATTAA10.684931506849315No Hit
CTTTACTGCCTTTGTGAGGTCAAGTGACTTCAAACTTCTGTTCCGTGCCA10.684931506849315No Hit
GGCGACGGCACGGTCGTGGGGGTCGGCCGGCAGGATGGACGGCCCGTCGA10.684931506849315No Hit
GATGCTTTATGTTTGACTGTTTATATCCAACTACCACATCCAAGATTATT10.684931506849315No Hit
GCGGCTGAGCTCCCGCAGGATCCGGTTAACGGCGGCGATGCCGCACTCGG10.684931506849315No Hit
TGCCTTTGCAGAACACCTCGACGAGGTTCTTATCGACGCTAAGCTTGTTG10.684931506849315No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC10.684931506849315No Hit
GCAGGGTTCATGGATCTACTTGACTCCATATTATCTAGCTCCTTAAAATG10.684931506849315No Hit
TCACGCATCACAAGGGTCTTGTACTCAATCAAACTCAGAAGTAATGTGTA10.684931506849315No Hit
ACCCATCAATATCTCCAAAATCAACTTTAAGATGGCCAGGAACCAAAGGA10.684931506849315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGTTTC10.684931506849315TruSeq Adapter, Index 5 (97% over 45bp)
CTTTTCCTTAGATTACAGAAGCACCATAACACATTCCAAAAGTAATTGCA10.684931506849315No Hit
CGCCACTCAAATTACTGCGGCTCCTCTATTAGACCATGACAAAACATCAA10.684931506849315No Hit
CTCCAGCGCCTCAAACACAGCGTCGCTCGCCTGTGTGCAGAGGCCTTTGG10.684931506849315No Hit
CAGATAAGTTAGGTCCCACACCGGAAAATGAGCCGAACTCGGACACGATG10.684931506849315No Hit
GTCAGTCCAACAACCCCAATGGGTTCTTTAAGTACATACGACTTAAAGTT10.684931506849315No Hit
CGCCAGGGACGCGGGCATGACCGGCCGGCGTGGACCCGAACGTGAAGGTC10.684931506849315No Hit
GGAAGATTGCTTCAAGTGGTAGATGGCTTTGTCAGCTTCTCCTAATCTGA10.684931506849315No Hit
AAAACTTCAGCAGAGACTTCAGAGTCTCCAAACATACAAGACCAGCTGCA10.684931506849315No Hit
GCCCTGCGGGAGCACCATCAGGTCGCCGGCGTACAGCGTCTTGGTGTACA10.684931506849315No Hit
TTTTGCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGA10.684931506849315No Hit
GTAGTCAGACTCTTTCTGCCGTTGCGCTGCTGGATGCGCACATGCACATA10.684931506849315No Hit
GGGATAGGAACGGTACGTAAGTTTGAAGAATTCTAATGATGTACTTGTCA10.684931506849315No Hit
GAGGGTTCCTCCAGTATGACAGGTTCTGCTTCCATAGGCAAGCCACGCAT10.684931506849315No Hit
GGACGGCCTCAAGCTGTTGAGGAACCCGCCGGCGGTGACCTTGATCGTCG10.684931506849315No Hit
CACCAGACCGGTGGGGAAGACGAGTCGGTCGCGCCCCTAGGTGGTGGACG10.684931506849315No Hit
CCTCGTCGTTGGAATGGATAAGTCGTGCAAGTGCTGGCAGAGCAGGCTTA10.684931506849315No Hit
CAAATTATTAATCTAAAATACTGCACTGCATGTCCCACGTTCCAGAAATC10.684931506849315No Hit
GCTTTGATGTAGTCAGCAGCGTCACCATGGCTCTCTCTTGCTAATTTTGC10.684931506849315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTAAGG10.684931506849315TruSeq Adapter, Index 5 (97% over 47bp)
AGTCAATGTTAAAAAATATAAATAATTCCAAGTCAAAGATGATAGCCCTT10.684931506849315No Hit
CCCCACTTTATTTATTTATTGTAATACACGGCAGTACAAGTTGTTACAGT10.684931506849315No Hit
GTAGGGATGGTGTGGTTGGCCTTATGGAGCTCCTTGGATATGTGGTCATA10.684931506849315No Hit
GGTGAATACAATCGACGATGATTAAATCATGTGGTGAGGATGGGCGGGAG10.684931506849315No Hit
CACCCGTTTGCACTCCACATCTACGTCCAAGGTAGAAGCCTGCGCTTGGT10.684931506849315No Hit
TTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGG10.684931506849315No Hit
CAGCTCCTCCGCCGCACCGCCGCCGGATGAGTTGCGGCGCGTGGACAGCG10.684931506849315No Hit
GTTGCCGCAGAGCATTTGCCAATTGTTGGGACTGAAGTTGTTGTTCATGC10.684931506849315No Hit
CTTGGATCGGTACGGTTAACGGTTCTTCAGAAGCTGATGGGGTAGACGTG10.684931506849315No Hit
GCGAGAAAGAACTCCCGAGTCTGCGTCCCTCGCTCGGTATCGCCCCCATC10.684931506849315No Hit
TCCCGATGCCTTCACCTCTAGGCTGGCGGCCCGGCGACGGCGGCCGACGG10.684931506849315No Hit
CAGCAAAGTACACCACCATTCAAACAAAATAGTAGCCTACAGAGCAGAAA10.684931506849315No Hit
CATCACTGTTCTGAACATCAGTTTCTTCAGTGCCTTGTGGGCGATTCAGT10.684931506849315No Hit
CGACGGAGGAAGAATACAGCCTCTCTGCCATCGATCAGCCTGTGGTCATA10.684931506849315No Hit
AGTCGCGGTAGCTGCTCGCTGTGGCGGTGCCGAAATGGCCGCCATGCCTC10.684931506849315No Hit
GCTTCTCCCGCTGAGGCGGTGGCGGGGGGCGGGGGGGCAGGGGCGGGCGG10.684931506849315No Hit
GCTGCGGCTGCGGCTCCTGCTGTTTTCCCTCCCTCTGGGGCTGTCGCCAC10.684931506849315No Hit
CATAAAACGGGTGACATTATTTTTGCCATCCTGAATATTCTCAGCAAGAA10.684931506849315No Hit
GGGTTTCAGCGCCGTGCCGGGGGTGGCGAGGCCGCCATAGTGGAAGTCGT10.684931506849315No Hit
GGTTTCAGCGCCGTGCCGGGGGTGGCGAGGCCGCCATAGTGGAAGTCGTC10.684931506849315No Hit
TGTAAGTTTGCCAGTTCACCCTCCAGACCAGCATCACAACCAAATGGTTT10.684931506849315No Hit
AAGAAATTTACGGTGGAAATCACTCCTGATGGTGTCGTTGATGAACCGTT10.684931506849315No Hit
TCGTAGTCGAAGCCGGTCCTGGGCAGGTGCTTGAGCCCGAAGAACTGCTT10.684931506849315No Hit
AGGGGCACGTCGCTGTTGCCGCCGCCGAGGAACGCGTTCCACAGGTTGTC10.684931506849315No Hit
CTTTGTTCATATGTTTGCTTTTTGCTATTTAGTAAAGTAGGTACAGTATC10.684931506849315No Hit
CTCGACTTGGCGTCCCATGCCGTACGTCAGAAGAAGACACCGGCCACCCG10.684931506849315No Hit
CTTCAAAGCAAGCATCATTCAAATCAAAATCATGTACCCGCCTTTTCACT10.684931506849315No Hit
CTCGATGTGCATGTCCATCCTGCCCCGCCGGATCAGCGCCGGGTCCAGCT10.684931506849315No Hit
TCACATCCTTAGCTACTGTACAAACATTTGCCTGTGCAACGTTTCTAGTA10.684931506849315No Hit
CCACTCAGAGCTATGCTCTGTTTCTGATGAGAAGCCAGCAGGACCAACCA10.684931506849315No Hit
AGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGC10.684931506849315No Hit
TGAGGATGTGCTCGACCACGGCGGGGTCGCACGTGTATATCTGCCGCCGG10.684931506849315No Hit
ATCAAGATTTTGCAATATTGCCTGGCAGATGGGATCATCTGGAATTCGGA10.684931506849315No Hit
CTGGAGGTCGACGGCCTGCTCTTCCTGTCGCCGCGGCCGGCTTCGGGGCC10.684931506849315No Hit
CTGAAATAGATTTCGCGGAAAACCAGCTATATCCGATCTTGGTTGGCCTT10.684931506849315No Hit
TTCCAGACCAAATGATCTTTTCCGATGCTACACACCCACGCTATCTATGT10.684931506849315No Hit
TGTGTTATAAAAAAATGCACAGTAGAAAACTTTTCTGCGAGTATATTCTT10.684931506849315No Hit
GGAAAAACAGATTTCAACTGCAGAAAAATAAAATCATCTAATACAAATCG10.684931506849315No Hit
CCGTTGCTCCATTGTTTGCGATCTCCTTAGGCATGTCAACAAAATAGTGG10.684931506849315No Hit
CCATCCTGGCGAGCTGCACGGCGAAGTGGCCGACGGCGCCGGAGGCCGCG10.684931506849315No Hit
GCAGGCGGTGCTCGGGCGCCAGCCGGTAGTCCACGGACATCACGGCGCAG10.684931506849315No Hit
CTCCCACGAGTGCAACAGTCTTGCCGGAGGGGATGTGCAAGGTGAAATCG10.684931506849315No Hit
GGCGATGGCGCGGGCAACACCCAGGGCGATGCGGTAACGGGTGTGAAGGT10.684931506849315No Hit
GCTGCATTGGAAGCGTTATTATTAGCCAGCTTCTCCTGGTTGTCGGCGGC10.684931506849315No Hit
GGGTATGCGTCTTCAGCATAAACATCATGTTGCATCATATCATCGCATCA10.684931506849315No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers