FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25752332
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1303860.5063075452739582TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG170350.054.36351845
CGTATGC176550.052.41475746
CGTCTGA182850.052.29344616
CTCGTAT176300.051.77440644
TATCTCG178900.051.589241
ATCGGAA186750.051.3196032
GATCGGA193250.049.375621
TATGCCG187200.048.8905448
GTCACAC193000.048.87422629
TCGGAAG197800.048.5593073
GTATGCC190600.048.42248547
ACGTCTG207150.046.24357615
AGTCACA207550.045.58305428
TCACACT207650.045.47673430
CACGTCT214900.044.77149214
CACACTG213000.044.43100431
ACACTGA215700.044.08577732
ACTGATA215500.043.99676534
CACACGT221800.043.3321912
GTCTGAA223000.043.0195317
Produced by FastQC (version 0.11.5)