FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC35.555555555555555TruSeq Adapter, Index 4 (100% over 50bp)
ACTGGGTTGATGCTTGCTGCCTCCAGCTTCAGCAGAAGCTCGTTCTTTTT23.7037037037037033No Hit
TTGGCAAGTGGCGGGATGCTCTGCTCGTTCCTGAAGTAGTCGTCGGGGTC11.8518518518518516No Hit
ACAAAAGTATAAGTTTGAAATATATGCTAGCTCAAATGACCATCCTCAGT11.8518518518518516No Hit
CGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGA11.8518518518518516No Hit
GTTTTCAATGTACACCCAATCCCATTCACGAAAAACTTTCACCTCTCCAT11.8518518518518516No Hit
CACACCACTGAGCTGGTTGTAGCTTAGATCCAATGAAGTTAACTTTGAAA11.8518518518518516No Hit
CCGTGCGGAGCGTCTCCGGCGTCAGCAGACGTGGCGCCGTCTCCGGAAGG11.8518518518518516No Hit
GCTTAGTAACACGCTAACTGTGCTGAAGTCTCCAGCTTGAATTGCATGCC11.8518518518518516No Hit
CGAGGAGCTCTCCCCACGGCCGGCGATCGGACAGTGAGCGCCGCGCCGTG11.8518518518518516No Hit
GTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCT11.8518518518518516No Hit
GTCACTACCTAGTGACACTACGCATTGCCTAGCAGATACTGCAGCAGCAG11.8518518518518516No Hit
TTGTCGGCATGATACTTGTTGTCACCTTCCAGACGCTCCACTTCTACGTA11.8518518518518516No Hit
TACACGGAACCGACTAGCTCTATTGGACGAACCTTGAGTAAAATCTGAAA11.8518518518518516No Hit
CGAAGTCCTGAAACGCGGCGCGCCCGCTGGTGAACCGCTTCATCTGGCCG11.8518518518518516No Hit
CGTCCTCGGGGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTC11.8518518518518516No Hit
CATTGTGAAAAAAAAATGTTCAACATCCTTAATCATCAGGGAAATGTTCA11.8518518518518516No Hit
CTCCTTGCCAAAAAAAAAGGTCTAAAATTTCCCTCCTTGTTTGTCGTCTC11.8518518518518516No Hit
TGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT11.8518518518518516No Hit
CTTGGCGATCGCTTGCGAGCTGGAGCCGGTCCTCTCCTTGAGCGCCGTGA11.8518518518518516No Hit
GGCGGAACAGCCCCCTCGGCGACGACCGCTCCTGATCGGCGCCGGCTCCT11.8518518518518516No Hit
GTTCATCGTATATCTTTTTGATACACTCATCATATCCGTGGCGTGACTTT11.8518518518518516No Hit
CTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGAC11.8518518518518516No Hit
CTGCCGTTGGCGATTTTTTTTACAGAACTGACATCATGCAGACGGGGTCA11.8518518518518516No Hit
GCCACATGCCGCACTACCACACTTCGTGAACATTACACACAATCCCTCGC11.8518518518518516No Hit
CCAAGACCTGTGCTGGCAAGAGAGCTAGGCTCTTACTCTCACACTGTTGA11.8518518518518516No Hit
ATCCTCTGAAAAATCTGTTGCCGGCTCATAGAATCTGGGTACAGGCATCC11.8518518518518516No Hit
TGAGATCAAGCTCAGCTGCTCCAATGTGACACTTCCAAAGAGGACACTCC11.8518518518518516No Hit
ACCTATTTTATAAAGTATATTTTATATTCTCCTTTCGGGGTTCCGGCTCC11.8518518518518516No Hit
CTGGCATGAAAACTATTCCTTCCTGTGGATTTTCACGGGCCGTCACAAGC11.8518518518518516No Hit
CAAGATAATCAATCAAATCAAGCAAATTAACCAACCAGACCACGTGACCG11.8518518518518516No Hit
ACTCTATGGGTGCTGTGCTAACATGAGCAAAGTTCTTCCACTTGATTGGC11.8518518518518516No Hit
GTGTGCCGGAAAAGGCTGGGTGTACTGATGAATGAATGGACTGAGGATGA11.8518518518518516No Hit
GGGAGGTCAAGCACGCTGCACTTGAGGTGGGGGAAGGCGGCGGCGATGGT11.8518518518518516No Hit
GGACAGCCTTATGGCACAGGGCACAAAAATGCTTCTGGCATGCCCAACAG11.8518518518518516No Hit
AAAAACAAAAAAACAAAAAAAAAAATAAAAAAAAAAAGAAAAGAAATCTA11.8518518518518516No Hit
CTTACCAATACTCTTTCCACCAATGCAAGCGTGCACTTGGACGTTGAGGT11.8518518518518516No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG11.8518518518518516No Hit
TACTTAAAAGAAAAAAGCTCTTAAAACAAATCTTTATGAAAAATAAAGTC11.8518518518518516No Hit
CAGGCACTCCTCCTCCTCCTTCCACACGATGAGCCTGATGGCGGAGCGCG11.8518518518518516No Hit
AAGAGATCAAGCTCCGGTGGTAGGTGGTATACATTGCATGAAGGCAAGAA11.8518518518518516No Hit
TTAGTACATGCCTGTCTTAGTTAGGGTTTTACTGCTGTGAACAGACACCA11.8518518518518516No Hit
ATTACCATACCTTGAAGCTTCCTGAAGAATATCTTCCTTAAGGCGAAACT11.8518518518518516No Hit
CTCTGAGGCAGCACAATGTCATCCATACTGAGCGCAGCAGCAGAGGCTAT11.8518518518518516No Hit
ATTTACTGAGGGGCGACCGTTGGCGGCACGTGACCAGGCAGAAAGGGGAG11.8518518518518516No Hit
CGAACCCTCGGCCCACCACGTAGAAGCTGAAGCCGTGCAGGTGCATGGGG11.8518518518518516No Hit
TAGCTTACATCTTACAGGCTACAGCACAAACACAAAACCTAATAACCTTG11.8518518518518516No Hit
GCTCCACCGCTTGTGCGCGCCCCCGTCAATTCATTTGAGTTTCATACTTG11.8518518518518516No Hit
GTGGAGATGAAAGATGCTGAAAACGCTGAAGACGAGAAGAAAGAGGTCCC11.8518518518518516No Hit
CCAACGACACCACCACTGGAAGACTAATGTTTGGAGTAACCATCTACATA11.8518518518518516No Hit
CTCCGCCTTGAACGCCACCACGGCGTCCCGCGCCACCCTGCCGGGGTCCC11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCA50.070.014
GAGGTGG50.070.024
TGCACTT50.070.017
GAGGTCA50.070.03
ACGCTGC50.070.013
TCAAGCA50.070.07
GCGATGG50.070.043
CACGCTG50.070.012
ACTTGAG50.070.020
ACCATGG50.070.063
GGCGCCA50.070.057
GTCAAGC50.070.06
GCGGCGC50.070.055
GCGGCGA50.070.040
ATGGTGG50.070.046
GGCGATG50.070.042
ATGGCCA50.070.066
CGCCACC50.070.059
CCATGGC50.070.064
AGGCGGC50.070.035
Produced by FastQC (version 0.11.5)