FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-3357-CHX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26699469
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG99350.042.5925928
CGTCTGA103550.041.13435716
ATCGGAA114650.037.399322
ACGGTAG113550.037.3266332
GATCGGA119550.035.953931
ACGTCTG127100.034.0632415
TCGGAAG126700.034.0361633
CACGTCT134900.032.0159714
GTAGCAT135400.030.55347435
CACGGTA140750.030.36173831
CACACGT143550.030.0142312
CAGTCAC143000.029.86060527
GTCTGAA146950.029.41456417
CGGTAGC147750.028.80533
GGTAGCA147400.028.80216434
GCACACG171500.025.42887311
CGGAAGA178150.024.9925334
AGCACAC187850.023.77465810
TCGTATG49950.023.54320543
TAGCATC164700.023.16299236
Produced by FastQC (version 0.11.5)