FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTTAATAGTTTTTTCTCATCCTGTGCGTTATGCAGGTGAGCGCTGTA11.5625No Hit
ACTACTAACTAAGGCGAAAACGATCGAACAGGGCTCCGCCTGACCCCGAA11.5625No Hit
CAGTATTCCTCTTTGATTGCCAAATAACTGTCATGATTTGGAACCCTATT11.5625No Hit
TCTACCATAACCATCATATGACTCAAACTCATCCCTAGTTTGCTTCTCTT11.5625No Hit
TTAAAAAAAAAAAAACCTACACACAAACAAGTTAGTGAAGCAAGCAACCC11.5625No Hit
AGGGTGGCGAGTTGGGAGGACACCTCGCGGCCGATGCCGCGGGAGCCGCC11.5625No Hit
GGCAGCATGTAACACTTGTCCATATCAGCGATAATCGTGAGACATTTCCA11.5625No Hit
GCTTTTTCTTCTTTGGATCTTCTTCTTTCTTTTTCTTCATGCCAATTTCC11.5625No Hit
GTCGTCTGCAAAGGATTCAGCACGCCGCCCGTTGGGAAGGGAGCTTCGAG11.5625No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC11.5625No Hit
CAATTGACATGATAACTTTAAATTTCTTATCAGAACAGGTATTCTTCAAA11.5625No Hit
CTTGGAGCAGGGCGGCGTGGTGAGGGAGCCCATGTAGCGGTAGTAGGCGC11.5625No Hit
TATGAAAAAAAAACCCTTTGGACAAAAGGTAGATTATGTTTATAAATTAT11.5625No Hit
GTTTGCTTTTTGCTATTTAGTAAAGTAGGTACAGTATCCAACATAGACTG11.5625No Hit
TGCATGCATGTACTAATCAGTCTTGGTATCCTCAGCTCAGTCGAGTTTAG11.5625No Hit
GGCAGTACTGCTTGTAGTAGCCGACGCGCGCGTTCATCTGGGCGGGGTTG11.5625No Hit
ATGCAAGATTAAAAAAAAAAAAAAAACTTTCCAGAATATCTAAATAAGGG11.5625No Hit
CTTGTCCTTCGCATCACGTGTAACGAACCACATCATGCTCCGACTCGATT11.5625No Hit
ATAAGTTACACGAAGGGAAGAATACATACAGACGGCACCCAACACTAACC11.5625No Hit
TGGTGGTGGAGGCTGAGCGGCGGCAGGCCGACGCGCAGGTCAAGGCTCAG11.5625No Hit
TTTATATCCATTGCCAGTCTTCTCATTTTTTTCTGAAATCTTTTATTGAT11.5625No Hit
CCGCAGGTACAGCCGCCGGAGCGTCTCGACGATGAACACGCCGCCGTCCG11.5625No Hit
CGAAGATCTGATGCTGCTGATCTCTGAACATCTATGGAATCACTTTTCAA11.5625No Hit
CAAAAAAACTTTTAAAAAAAAAATCTATTTAGTGTGTTGGGGACATGGCT11.5625No Hit
CAACAACTCAAAAAAAGAACAAAGGGCGGCTACAACAATACTAGCTTTAG11.5625No Hit
GGGGCTGCCATTTCGAAACTCTTTTTTGTCTTTCTGCTTTTGACTTATGG11.5625No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA11.5625No Hit
CTCCCCTTGTCCGTACCAGTTCTGAGTCGGTTGTTCGACGCCCGGGGAAC11.5625No Hit
TCCTGGATTCATCAGATATAACTGCTGATTTGCGCTCACTGCCCATCATG11.5625No Hit
CAAAACAAAAAAAAACCAAAAACAAATAAATAGATTTCTTAAAACCTTGA11.5625No Hit
GGACGGTCCTGGTCTCACTCCTCGGCAGAGTTATCGCATCATGCTTTATC11.5625No Hit
GCAATTTCTTGTTGGGATGTGTAATCGTTGACCACGATATTAGTGCTTCC11.5625No Hit
GCCTGGGGCAAGATGCACAGCAGCGACCTTGAGCCACTCTGAGCCGAGGT11.5625No Hit
CCAGTTTTTCTACTCTTAAAAAAAAAAGGATACTTGTTCATGCTGTCACT11.5625No Hit
CTGCCTTCAAAACGATGTCAGATATATCCTTCAAGCCAATGATGCAATCC11.5625No Hit
CGATCCATTTTATATGAATTATTTAAAAGGACGTAAGGAAAAGGGAGGCT11.5625No Hit
CCTAATAAAGCTGGTAGCGGCGAGGGGAAACACGAATTTGCAAATCACTC11.5625No Hit
CCTGTGAGCTATGGTTATAACCGTGCAACTTGAGAACTGCTGCCTGATTA11.5625No Hit
TGCCTTGCACCTGTTCGCTCATCCTTCTCCACCATTCCATGGCCTTCCCT11.5625No Hit
GAAATGGTCATCACTTGCAGTTGGATGAAATTCTGGGGTTCTGAACTCTC11.5625No Hit
ATGCTACTTCAGAAGGCGAGATAACTATCATATCTGCTACACTGACTTTA11.5625No Hit
GCCACAACTGAGCATGTGATCAAGAGGAAGGCTGATAACCAAAGGGTTTC11.5625No Hit
TCAAAGAGCGTTGTTCTTTTTATCTGAAGACGGTAAGGGCAAAGGAAGCA11.5625No Hit
GGCGAGTTCTGTTAGATCCTCGATCTGAATTTTTGACTCCATGGCCTTTG11.5625No Hit
GTCGCGATCACTAAGTTAGTTGTAGTCAGCCTTGTTTTACAAGCCAACGA11.5625No Hit
CACCAATAACCTTGTGGCCTGGAAGTTCACCACCTTCACCAGGCTTGGCA11.5625No Hit
TTTTCTTCTTGATCATTACCAAACTCTTCTGATTTACCATCTAGTTGCTC11.5625No Hit
CGGAGCTGCTCCTGGCCGGCGCTGTCGAGCTCCTGCGACATCCATGCGCC11.5625No Hit
ATTCCCTTGTCAGCCCACCAGCATCAATTCCTTCTTCTCCTTGAAAATGA11.5625No Hit
GGAGATAGGACGTCCTGAAGGATTGTGCAAAATTTTCAATTGTATGGTAC11.5625No Hit
CAAACTATCAAATTGGAGAGCCGAGAAGTTTTCTGAGACATAGAAGTCCA11.5625No Hit
GCTCACACCAGGGATAGAAAAAAAAAAAAAAAAACCTTGGCACAGGGCGC11.5625No Hit
CACCCTTGTAGGTGCCGAGGGTAGCCTCAGAGTTGGCCTTGCACCTGGCG11.5625No Hit
TGCGCCGTAGCAGCCGGGCTCGGCCACTGCCACGACGACACTCATGGAGC11.5625No Hit
CTCGTAGAGTGCCCATGTCCTCTCAAGGAGGAACTGGTCCTCGCTAGGTA11.5625No Hit
TGGTTCTCCAGCTGGAACCCACCGATGACGGCCGCCGGCGCTTGCCCAGG11.5625No Hit
CGACGACGATGTTCTCTTGTTCATGATCCTCCCAGCTAAACACTCTGCCA11.5625No Hit
CCACCACCGACGTCAACCAACGAGTCGATCCCACGAAACGTCTCGCCGCA11.5625No Hit
GCTGAGCACCAGCAGGTCGCTGTACGTCGGGCAGAAGCGCAAGGGCAAGT11.5625No Hit
TCCAACAACAGGCTAACTTACAACAGCACCCCGGTCTGATATAAACGTTT11.5625No Hit
AAGGCATCGCAGTTGACGTGGTGGTGGTTCCTGAAGAAGTTGCATTGCTC11.5625No Hit
GCGGCGGCGGCCTCGGTGCCCTTGAACCTGGTGACGAGGTTCTTCCACTT11.5625No Hit
ATCATCATGTGGACCCCAGGACTTGCTCCGTCTGTAATGAAAATGTCATC11.5625No Hit
GTTTTCTTTCAAAGGTGAGCAGACCATCTGGGGTTTAGGCTTCTGTGAAT11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC50.070.051
GAGGCGG50.070.048
ACGCCGC50.070.022
AGCTTCG50.070.042
CAGCACG50.070.018
AGGATTC50.070.012
CGCCCGT50.070.026
TGCAAAG50.070.07
CGGGGCC50.070.052
GTCGTCT50.070.01
CACGCCG50.070.021
CCGCCCG50.070.025
GCGGCGG50.070.066
GCGGCCG50.070.069
TTCGAGG50.070.045
GAGCTTC50.070.041
GGATTCA50.070.013
GCGCGGC50.070.064
AGGCGGG50.070.049
GTCTGCA50.070.04
Produced by FastQC (version 0.11.5)