FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23204840
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT990500.42685060530475544TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG157650.052.8404828
TCGTATG143850.052.62658345
TATGCCG142050.052.2587348
CTCGTAT147900.051.4222444
CGTCTGA164400.051.24323316
CGTATGC149900.050.0123546
ATCGGAA170650.049.8022082
ACGTCTG170400.049.5209515
TATCTCG160450.049.01429741
GATCGGA172950.048.8156661
GTCACGA173200.048.1771629
CACGTCT175650.048.1405514
TCGGAAG182200.046.7606243
GTATGCC162000.046.42809347
ACGAGTG182050.045.9095532
GTGGATA184900.045.01262736
TCACGAG188200.044.50470430
CACGAGT189000.044.33245531
CGAGTGG194550.042.86987733
CACACGT199600.042.46992512
Produced by FastQC (version 0.11.5)