FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23689895
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT327560.13826992479282835TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA81600.050.99767736
ACCGTAC93250.044.40128732
CGTAATC90200.042.10007538
CGTCTGA102900.040.7139316
CACCGTA104000.039.9126931
ACGTCTG106100.039.65075715
CACGTCT110800.038.15834814
GATCGGA110300.037.9846881
ATCGGAA111500.037.7960242
TCGGAAG119200.035.677733
CCGTACG118850.035.1023933
TCGTATG59300.034.29114545
CACACGT132450.032.15929412
AGTCACC130250.032.1396828
TATGCCG60100.032.02953348
AATCTCG89650.031.97372441
CTCGTAT71300.031.85786244
CGTATGC64150.030.11645746
GCACACG145100.029.62093711
CAGTCAC143650.029.43394327
Produced by FastQC (version 0.11.5)