FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0808-183-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25998523
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG89550.046.11866433
TCGGAAT93450.043.8941837
AGTCACG97700.042.27345728
CGTCTGA102150.040.94450816
GTTTCGG107200.038.85191734
AATCGGG64150.038.51833341
ATCGGAA110000.038.2805632
ACGTCTG110350.038.0921915
TTTCGGA112000.037.15551435
TTCGGAA112250.036.94804436
CACGTCT113750.036.92277514
CACGTTT114600.036.49585731
GATCGGA114650.036.452781
TCGGAAG119300.035.5607533
ACGTTTC118750.035.51515632
ATCGGGG74400.031.23606342
GAATCGG83400.030.55095540
CGGAATC122450.030.32612238
TCACGTT138200.030.23964130
GTCTGAA141250.029.98222717
Produced by FastQC (version 0.11.5)