FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6483977
Sequences flagged as poor quality0
Sequence length110
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1217131.8771349744146224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT65830.10152719542342609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG93600.075.829842
GTATCAA108700.063.9705929
AAGCAGT129750.055.4329221
GGTATCA128450.054.4624378
GCAGTGG141800.049.946963
GTGGTAT149500.046.8873256
CAGTGGT150950.046.672034
AGTGGTA151650.046.4235765
TGGTATC181600.038.5346267
ATCAACG103950.036.13837410-11
TACATGG103250.035.6920322-23
CATGGGG67100.035.5957424-25
CAGAGTA99550.034.9296618-19
AGAGTAC110900.033.90530418-19
GAGTACA103200.033.4043920-21
AGTACAT112100.033.32271220-21
GTCCGGC17700.032.9294830-31
GGGTCCG18250.032.92756328-29
GGGGTCC16800.032.82744228-29
CCGGCGC22300.031.0432932-33
Produced by FastQC (version 0.11.5)