FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n02_CRISPRscreening_batch1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n02_CRISPRscreening_batch1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31456364
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1004710.3193980079833766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC321450.10218917863488608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1232300.083.915941
AGCAGTG1290350.080.000412
GCAGTGG1419700.072.7234953
GTGGTAT1492150.069.1606146
GGTATCA1504500.068.525368
CAGTGGT1526550.067.626174
AGTGGTA1547150.066.858835
GTATCAA1584950.064.9191069
TGGTATC1635750.063.1526537
GCAGAGT1464400.038.67698316-17
CAACGCA1465050.038.67457212-13
ATCAACG1526300.037.22427710-11
GGGTCCG234600.036.9797428-29
CATGGGG999500.036.1274724-25
TACATGG1542250.036.06088322-23
TCAACGC1469900.034.98562612-13
CAGAGTA1482350.034.7127618-19
GTCCGGC247750.034.18792330-31
AACGCAG1505800.034.18208314-15
AGTACAT1658350.033.96840720-21
Produced by FastQC (version 0.11.5)