FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n02_CRISPRscreening_batch1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n02_CRISPRscreening_batch1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32136439
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1010190.31434410016616965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC325090.10115931015256545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1243750.084.4981461
AGCAGTG1308100.080.1470952
GCAGTGG1445300.072.55513
GTGGTAT1515050.069.1170046
GGTATCA1524800.068.508248
CAGTGGT1555950.067.352694
AGTGGTA1569800.066.7911455
GTATCAA1598450.065.224849
TGGTATC1647050.063.5682757
CAACGCA1480850.038.74864212-13
GCAGAGT1488600.038.5459316-17
ATCAACG1544700.037.19586610-11
GGGTCCG233850.036.8864328-29
TACATGG1557300.036.1588822-23
CATGGGG1011450.036.05974224-25
TCAACGC1492950.034.92297412-13
CAGAGTA1505800.034.69497318-19
AACGCAG1522300.034.28308514-15
AGTACAT1681950.033.83435420-21
GTCCGGC246750.033.73596630-31
Produced by FastQC (version 0.11.5)