FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n02_CRISPRscreening_batch1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n02_CRISPRscreening_batch1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37776100
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1208770.31998274040994173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC385000.10191629098821743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1468300.085.109211
AGCAGTG1551450.080.5379642
GCAGTGG1707100.073.0825043
GTGGTAT1798700.069.33376
GGTATCA1821000.068.426028
CAGTGGT1847750.067.526494
AGTGGTA1858300.067.242135
GTATCAA1918700.064.900969
TGGTATC1972550.063.3414887
CAACGCA1764300.038.7839412-13
GCAGAGT1769600.038.60909716-17
ATCAACG1837850.037.3138610-11
GGGTCCG280750.036.6514828-29
CATGGGG1200450.036.15616224-25
TACATGG1857950.036.11338822-23
TCAACGC1779400.034.95202312-13
CAGAGTA1786600.034.7683118-19
AACGCAG1816950.034.25815614-15
AGTACAT1997100.034.0186320-21
GTCCGGC299550.033.58748230-31
Produced by FastQC (version 0.11.5)