FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n02_CRISPRscreening_batch1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n02_CRISPRscreening_batch1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35080988
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT1111340.3167926741401924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC351540.10020812412694877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1379650.083.845041
AGCAGTG1444850.080.063732
GCAGTGG1593950.072.451233
GTGGTAT1668600.069.163486
GGTATCA1693250.068.0045248
CAGTGGT1711200.067.443184
AGTGGTA1737950.066.5129555
GTATCAA1784150.064.568929
TGGTATC1830100.063.1509327
CAACGCA1643050.038.67600312-13
GCAGAGT1647200.038.49580816-17
ATCAACG1712150.037.19679310-11
GGGTCCG258750.036.96489728-29
CATGGGG1124750.036.10334424-25
TACATGG1734250.035.88967522-23
TCAACGC1656100.034.7456812-13
CAGAGTA1663400.034.57590518-19
GTCCGGC270750.034.30862830-31
AACGCAG1688200.034.1169214-15
AGTACAT1866200.033.81138220-21
Produced by FastQC (version 0.11.5)