FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6483977
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG647470.9985692423029877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA21400.019.9373423
TTCGCAT18750.019.7054944
GCGCAGA5550.019.6149925
CGCATAT18900.019.4325876
TCGCATA18850.019.4257975
CGCAGAG6500.018.9474456
AGCCAAA7700.018.8507735
TGTGCGG11750.018.7258931
GGGATCC7350.018.709971
ACATGCA8650.018.6961271
GTGACAG5900.018.4515344
GAGAGAC3400.018.4349465
GCATATG20100.018.4217687
GTGCGGC5500.018.3939252
GCCAAAC6250.018.2978176
AGGTGTA6150.018.237884
TATCGCC8150.018.2232841
GAGACGA3300.017.9794887
TGAACGA4900.017.9532552
GAAATAG7650.017.8241793
Produced by FastQC (version 0.11.5)