FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n01_CRISPRscreening_batch1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n01_CRISPRscreening_batch1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31456364
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA292000.019.9026853
CGCATAT257700.019.747126
TGTGCGG142700.019.2293361
TTCGCAT264950.019.2275874
TATGGCT266900.019.10574310
TCGCATA267850.019.0563135
GCGCAGA68750.018.9928685
CTTTCGC268450.018.9851262
AGCGCCA104650.018.8164251
GCATATG270200.018.8154097
TGCGCAG101650.018.678734
GGGATCC87300.018.5718211
ATATGGC276050.018.500359
TCGCACC108450.018.1972355
GCCCGAA79800.018.193151
CGCAGAG88750.018.0966036
AAAGGCG62800.018.0404199
TATCGCC120600.018.0254361
ACATGCA122500.018.02441
GTGCGCA92400.018.0008813
Produced by FastQC (version 0.11.5)