FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n01_CRISPRscreening_batch1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n01_CRISPRscreening_batch1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32136439
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA305300.019.8896063
CGCATAT269550.019.6911856
TTCGCAT273900.019.4146024
TATGGCT277050.019.1958910
TCGCATA278600.019.1107675
CTTTCGC278850.019.0977572
GCATATG280800.018.9277867
TGTGCGG146800.018.9238281
GCGCAGA71400.018.7964365
TGCGCAG108050.018.7737064
AGCGCCA111350.018.6520861
GTGCGCA95650.018.5393263
ATATGGC290100.018.3627059
TCGCACC110950.018.3325775
GGGATCC89050.018.3172381
CGCAGAG91250.018.2156836
GCCCGAA78600.018.2039871
TATCGCC123350.018.0956651
ACATGCA130850.017.9584921
GGGCCAA67150.017.9365169
Produced by FastQC (version 0.11.5)