FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n01_CRISPRscreening_batch1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n01_CRISPRscreening_batch1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37776100
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT314650.019.9311146
TTTCGCA357850.019.9288983
TTCGCAT325850.019.320324
GCGCAGA83400.019.2440975
TATGGCT327300.019.226310
TCGCATA329300.019.1012025
CTTTCGC331300.019.0524752
TGTGCGG174350.019.0155931
TGCGCAG123750.018.969574
GCATATG331050.018.9686417
AGCGCCA133950.018.8424841
ATATGGC340200.018.5166599
TCGCACC134000.018.4051385
CGCAGAG106550.018.356366
GGGATCC106550.018.2540781
ACATGCA149050.018.1668641
TATCGCC142300.018.1622661
GTGCGCA111850.018.1454893
GTGCGGC96950.018.1204682
GCCCGAA90100.018.1050721
Produced by FastQC (version 0.11.5)