FastQCFastQC Report
Fri 28 Jun 2019
HMGCKAFXY_n01_CRISPRscreening_batch1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGCKAFXY_n01_CRISPRscreening_batch1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35080988
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA324150.020.0157093
CGCATAT290600.019.6312776
TTCGCAT296550.019.2708054
TATGGCT296500.019.24610910
AGCGCCA122500.019.1308751
CTTTCGC299950.019.1074332
TCGCATA299050.019.0986425
GCGCAGA74800.019.0008285
TGTGCGG155850.018.844051
GCATATG303250.018.7886967
TGCGCAG115400.018.7408774
ATATGGC309050.018.4538789
CGCAGAG93950.018.3595036
TCGCACC124450.018.34155
GGGATCC101450.018.2285651
GCCCGAA87250.018.1799951
ACATGCA140300.018.1787321
AAGGCAG66250.018.146887
AAAGGCG70600.017.9791349
AGAGCCA117600.017.97873
Produced by FastQC (version 0.11.5)