FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n02_2a_c2d5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n02_2a_c2d5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77981283
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3498730.4486627900184715No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1725400.221258221668397No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1468420.18830416011493425No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1349880.17310307654209794No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1220730.15654141007143985No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1186770.15218651891121104No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1061010.1360595721411765No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1051160.13479644852727032No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1015160.13017995613126807No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1011170.12966829489071116No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC991010.12708305914894988No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC975920.1251479794196256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG973900.12488894290184993No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT971640.12459912976810089No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG827230.1060805834651374No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA794750.10191548143674425No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG790760.10140382019618734No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC786970.1009178061356082No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG784220.10056515741091358No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC783630.1004894982299791No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT780700.10011376704330448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG313950.054.1142923
GGCCGAC198350.040.698591
GTCCAAT241600.040.214851
GAGTCTA415550.040.213032
TGGGTCT247850.040.1895031
GGAGTCT422050.039.7739871
CCGGCAT161050.037.817211
AGTCTAA275500.036.4097483
GTTGTGG289250.036.3061071
CGCGGAT94000.035.7315251
GGGTCTG495800.035.562912
CGAAGGA1770650.035.4354678
CCTGGCC459950.035.098565
TCAAAGG299150.034.3875967
AGTCTAG250650.034.3847735
ATCGAAG1840250.034.310776
TCTAACG299800.033.378945
TGGCCCG489950.032.9771237
TCTGGAG527700.032.3237345
GTCGTCT857800.032.2235071
Produced by FastQC (version 0.11.2)