FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n02_2a_c1d5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n02_2a_c1d5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76067876
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT3153040.4145034889629362No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2363020.3106462444146594No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1755660.23080176446625117No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1574370.20696910217395842No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1547890.20348800063774622No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC1538120.20220362140780687No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC1428120.18774285218638154No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC1380460.18147739526735307No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA1365620.1795265060378444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1287140.1692094045060493No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1268700.1667852537383849No Hit
GGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCC1242580.1633514783559883No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1214560.15966792605067612No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA1158650.15231791144004073No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC1145230.15055369759502682No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA1128240.14832016605800852No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1126820.14813349067351375No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1091100.14343768452270178No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT1090950.1434179652919453No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1079570.14192193298521968No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC1066980.14026683221705835No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1057250.1389877114486541No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC1050280.13807142452616925No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1001350.13163901145340248No Hit
CTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTC938130.12332801299723421No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA898850.11816420376980158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA883500.11614626915572086No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT867360.11402447992632264No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA856550.11260338069647166No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA846440.1112743045434843No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG835870.10988475608284369No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA817680.10749347069977346No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA792270.1041530330096242No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA777450.10220477301088307No Hit
GGCAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTG770720.1013200368576086No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT766030.10070348224262236No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC762670.10026177147367701No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC186650.060.665171
GTCGTCT855850.058.925791
CGGGGCA125400.052.1315881
GTCTGCA1029650.049.2743154
CGTCTGC1042400.048.790363
TCTGCAA1162300.043.8466645
CCGGCAT171950.042.7971421
GAGTCTA345100.041.611514
GGGCACT56800.041.367611
GGTCTGG295700.041.2711333
CTGCAAG1252250.040.6958436
GGAGTCT356750.040.3603553
GCAAGTG1328550.038.325648
TCGTCTG1297900.038.1860352
CGATGGG121550.037.7894552
TGCAAGT1360650.037.5325557
AGGGGCA87500.037.1500741
AGTCTAG285750.036.8579375
CCTGGCC469550.035.882385
TGGGTCT231150.035.2974851
Produced by FastQC (version 0.11.2)