FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n01_2a_t3d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n01_2a_t3d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72614426
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA4061900.5593792065505No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2081340.28662899573150935No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT2034630.280196389626491No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1725260.23759190770164593No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1542030.21235862967504557No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1503520.2070552757657273No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1463480.20154121992233334No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1421470.19575586812460655No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1331990.18343324782323556No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1304760.17968330425141693No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1286500.17716865241074825No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1262020.17379742146553634No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG1258580.1733236863980719No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1228580.1691922759259985No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1213210.1670756166274729No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC1164650.16038824021001008No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG1121190.1544032035727997No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1070200.14738118290709895No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1030030.14184922428499264No Hit
TGTGAAGTGAGTACGGTTTGATCCATGCAGTGTAACATTAAAATGAAAAC1027790.14154074563641114No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1020970.14060153832242647No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC985820.13576090238598043No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT952300.1311447397518504No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC947470.13047958266584658No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC939610.12939715312216335No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT900670.12403458232941207No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA863280.11888546774438456No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA847110.11665863749993699No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC816130.11239226761910918No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT810750.11165136800778401No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG790100.10880758046617348No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT788230.1085500558800809No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC768920.10589080467288965No Hit
GTCGGATTCCCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGG762350.10498602577950558No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC761080.10481112940285447No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA758550.10446271378637627No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC751780.10353039215651172No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT748550.10308557696235181No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA742830.10229785469900982No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG730330.10057643366897923No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG729200.10042081720786444No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA727240.10015089839035567No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT726990.10011646996975504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT102350.063.1742631
GGCCGAC96450.050.9136051
CTTGTTA249750.050.077891
GGGCACT37500.048.386141
GTTACGA264350.047.149164
GGTCTGG113600.047.0938573
GTCCAAT175500.045.2532841
TTACGAC276100.044.907985
TCCAATC180200.043.8733442
CCGGTCA106550.042.1006133
CCTGGCC575100.042.0430375
GTCGTCT732000.041.5100331
GGAGTCT263950.040.0184361
GCCCGGA612050.039.817659
GAGTCTA268000.039.386952
CGAAGGA1766800.039.1770178
CTGGCCC618750.039.1236956
ACCTGGC622800.038.753564
ATCGAAG1817700.038.261976
CGCGGAT71150.036.8364831
Produced by FastQC (version 0.11.2)