FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n01_2a_t2d5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n01_2a_t2d5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44810753
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2469960.5511980573055757No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1262310.2816980111894125No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1150340.25671070512919075No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA915800.2043705893538544No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG898910.20060140475657706No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC895400.19981811062179652No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC874680.1951942204586475No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC817850.1825119966183117No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT776680.17332446968699677No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC720380.1607605210294056No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT710170.15848205005615507No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG654950.14615911497849635No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT645180.14397883472299608No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG626980.1399173095796895No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT625420.13956917885312037No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC612130.13660337285561794No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA609310.13597405961912756No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT582800.13005806887467392No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT582110.1299040879763837No Hit
TGTGAAGTGAGTACGGTTTGATCCATGCAGTGTAACATTAAAATGAAAAC579610.12934618617098445No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG567020.1265365926789938No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG564870.12605679712635046No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG558000.1245236829651133No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG506820.11310231720497979No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT500430.11167632019037929No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA482300.10763041629762392No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT478680.1068225744834058No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC471500.10522028049829914No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC470820.10506853120723054No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG460160.10268963790900813No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA449930.1004067037213144No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA449340.10027503889524017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT80700.051.8393971
GGTCTGG103600.048.9991073
GTCCAAT153750.045.661151
GGAGTCT162950.042.2877851
GAGTCTA161700.042.2140162
GGCCGAC69550.041.9290731
GTCGTCT504100.038.6374051
TCAAAGG193950.038.1998447
CGAAGGA1159400.036.303138
GAGCGCT227600.035.9072723
AGTCTAG131800.035.7836533
GTTACGA162500.035.7586334
CCTGGCC266950.035.5775155
ATCGAAG1204950.035.1673476
TCCAATC199350.035.1443022
CTTGTTA169400.034.5568771
GGGTCTG147800.034.1995962
CGCGGAT52400.033.9408721
GACATCG1152800.033.6786543
TTACGAC175050.033.5240335
Produced by FastQC (version 0.11.2)