FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n01_2a_c2d5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n01_2a_c2d5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77981283
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3518930.45125315519622833No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1767790.2266941414646897No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1506310.19316301836172664No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1290680.165511511268672No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1273630.16332508917556537No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1263450.16201964771469585No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1193420.15303928764547256No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1111300.14250855554659186No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1077050.13811647597539528No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1061000.1360582897821776No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1042220.13365001958226308No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA926210.11877337283614582No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG884650.11344388883676099No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA883730.11332591180886314No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC856420.10982378938289589No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC855220.10966990630302915No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG852360.10930315162934676No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT849580.10894665582765546No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG849550.1089428087506588No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC827490.10611392479910851No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT814300.10442249327957324No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT804020.10310422822871483No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC798110.10234635406037114No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT781940.10027277955916677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG289450.055.9719853
GGCCGAC179350.041.311481
GAGTCTA404550.040.6523252
GGAGTCT408650.040.349741
TGGGTCT245950.040.1079521
GTTGTGG297650.039.285921
GTCCAAT234000.039.202191
AGTCTAG219400.039.0221525
CCGGCAT146800.038.797811
AGTCTAA256950.038.41943
CGAAGGA1706200.036.7330178
ATCGAAG1761200.035.802516
CCTGGCC499750.034.853715
GGGTCTG494050.033.5648352
GTCGTCT824550.033.419371
CGCAGGC360300.032.8748253
GACATCG1809050.032.8729483
TCTAACG301600.032.8513375
TGGCGAG357150.032.842515
TCTGGAG493300.032.6382335
Produced by FastQC (version 0.11.2)