FastQCFastQC Report
Thu 15 Mar 2018
HMF3FBGX5_n01_2a_c1d5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMF3FBGX5_n01_2a_c1d5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76067876
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT3969110.5217853065859234TruSeq Adapter, Index 7 (97% over 36bp)
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT3206250.42149855741995473No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2356710.30981672210750305No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1804290.23719473907750496No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1752090.23033244677424675No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC1571270.20656157140499098No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1500720.19728695987252226No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1294280.1701480398900582No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1246790.16390493143255375No Hit
GGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCC1242230.16330546681755648No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC1240760.16311221835614287No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1232480.16202371681838468No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC1218020.16012278297345914No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1199590.1576999468211785No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1193400.1568861998986274No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC1177060.15473811836155385No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT1129060.14842796451947732No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1128580.14836486298105656No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC1127160.1481781875965618No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC1125120.14791000605827354No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA1106440.14545430452139874No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA1106100.14540960759835073No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1047180.1376638937572018No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1016370.13361356375981892No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1009630.13272751299116067No Hit
CTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTC996440.13099353529997343No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT983660.12931345683952053No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG922990.12133768530621257No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA892730.11735965915493683No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA885330.11638684377095003No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA879230.1155849283868528No Hit
GGCAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTG846380.11126641685118172No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA830490.10917749300637762No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT814040.10701495070008266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT803020.10556624454717259No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT799180.10506143223980646No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC780910.10265962993366608No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA764870.10055098685810551No Hit
GTGAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAAT762360.10022101839678027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCT822500.060.3176081
GGCCGAC170600.058.414121
GTCTGCA1042250.047.9184274
CGGGGCA111550.046.604561
CGTCTGC1080700.046.2198143
GAGTCTA345350.045.327884
GGTCTGG258150.044.962933
GGAGTCT350100.044.609683
TCTGCAA1127200.044.562725
AGTCTAG255900.043.642315
GGGCACT54200.042.2462921
CTGCAAG1209350.041.5535356
AGGGGCA74600.041.3111
CCGGCAT159800.039.6972051
GCAAGTG1281050.039.2232068
TGCAAGT1305500.038.594877
CCTGGCC481000.038.158475
TCGTCTG1318200.037.138562
CGATGGG118900.036.6382642
CGGGTCT85250.035.221061
Produced by FastQC (version 0.11.2)