FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18500881
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC255550.13812855722924763No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG210350.11369728825346209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG126700.032.102795
GCTGACT74500.028.8998871
CTAGTTG71150.026.6173178
TACTCTA73250.025.904054
GACTCTA90600.025.6271044
CTAGCAG87150.024.778438
TCTAGTT84250.024.1177567
CTCTAGT85750.023.2138566
CTGACTC96950.022.2408752
GGACACC79750.021.93098829
ACCTCTA84200.021.59034317
GTTACCT87950.020.98326514
GACACCT83600.020.54951730
GTACTCT93550.020.5042933
CTAATGC88800.020.04488621
CCTCTAA96700.019.22767618
TGTTACC99050.018.84114513
CTCTAGC123700.018.4347536
TACCTCT99350.018.4020216
TTACCTC103750.017.98732615
Produced by FastQC (version 0.11.2)