FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17705649
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT368920.20836287898850814No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT364750.2060076984469759No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC294770.16648358950298855No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTG187840.10609043475333776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA131550.040.124324
ACTCTAG189500.039.8147745
TCTAGTT145050.037.0300837
CTAGTTG142550.037.0003368
TTGTACT144100.036.1562921
CTCTAGT149850.035.7076266
GTACTCT148800.035.542673
TGTACTC152600.034.545242
GCTGACT69850.032.1577381
TAGTTGT168700.031.5716769
ACCTCTA168050.030.7088417
GTTACCT172450.030.16518614
TGTTACC174950.029.67498213
CTAATGC178700.028.66634221
TACCTCT181300.028.54032316
TTACCTC183550.028.49116515
CCTCTAA181950.028.34405118
ATGCTGG178850.028.2201224
GGACACC177200.027.85886629
GACACCT182450.027.16998330
Produced by FastQC (version 0.11.2)