FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18786330
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT428170.22791572382684644No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC413310.22000571692289023No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT364560.194055997100019No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC216270.11512094166343292No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA208860.11117658425035652No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT206040.10967549276521811No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT198310.10556079872971465No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT197930.10535852399058251No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA197020.1048741292205556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG199850.039.4102335
TACTCTA126650.037.046564
GCTGACT83950.036.785791
CTAGTTG128550.036.119728
TCTAGTT137200.034.5982557
CTCTAGT139850.034.0426866
TTGTACT135900.034.0222551
GTACTCT142100.032.8487633
TGTACTC142850.032.7737772
TAGTTGT152550.030.5499889
ACCTCTA150650.030.47683717
GTTACCT154250.029.96737514
GACTCTA112450.029.5447944
TACCTCT161100.028.735616
GGACACC155450.028.60528829
CTGACTC108900.028.5762672
CCTCTAA161900.028.52961218
CTAATGC160750.028.51927221
TGTTACC164150.028.22336613
CTAGCAG112800.027.7070628
Produced by FastQC (version 0.11.2)