FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25017903
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC311680.12458278377688169No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG276550.11054083949402155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG149850.027.5115665
GCTGACT91100.026.9288561
GACTCTA116600.022.4572244
CTAGCAG112000.021.4367928
CTAGTTG84600.020.3469248
TACTCTA84950.020.305774
CTGACTC128650.019.5228062
TCTAGTT99600.018.9457287
GGACACC87050.017.8729929
CTCTAGT104600.017.2824256
GACACCT96050.016.44925530
ACCTCTA101650.016.22142817
GTTACCT106750.015.6401814
CTCTAGC165500.015.6339356
GTACTCT110050.015.5804843
CTATCGA154200.015.41357717
CTAATGC106100.015.05347621
TCTATCG159000.014.92629716
TGACTCT177850.014.82023
CGGGGAT41400.014.6682741
Produced by FastQC (version 0.11.2)