FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15256884
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC382400.25064095656754026No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT355740.2331668773256715No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC241890.15854482474927384No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT222740.14599311366593598No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA210270.13781975402054575No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT200760.13158650219795864No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT186650.12233821794804234No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC164010.10749901487092646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT76000.039.1329041
ACTCTAG134900.034.830145
CTGACTC100800.029.4336172
GACTCTA109000.029.1808074
CTAGCAG104300.028.7082658
TACTCTA58700.028.6209344
CTAGTTG61400.026.9677318
TCTAGTT70650.025.146367
CTCTAGT71900.024.181976
GTACTCT70850.023.664143
CTCTAGC132550.023.5797146
TGACTCT132950.023.0159873
GTTACCT72100.022.7263614
TGTACTC74100.022.7199462
ACCTCTA72300.022.5680717
TAGTTGT76150.022.1518759
TACCTCT77000.021.32479716
GGACACC73400.021.14967329
GACACCT74750.020.81344630
TAGCAGA144750.020.7096489
Produced by FastQC (version 0.11.2)