FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21593416
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT441050.2042520738728879No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC438070.20287202358348488No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT365430.16923214001897616No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC267220.1237506840047911No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA232110.10749109821252922No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT229410.1062407170778352No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT217960.10093817485848464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG212850.038.721565
TACTCTA135600.037.2734
CTAGTTG142700.035.655968
GCTGACT91200.034.2395861
TCTAGTT151450.034.121717
TTGTACT149850.033.341611
GTACTCT155350.032.5790373
CTCTAGT157000.032.5654376
TGTACTC157050.032.2268332
TAGTTGT169750.030.2787939
GTTACCT168900.029.30794514
ACCTCTA169200.028.99100917
GACTCTA120400.027.794684
TGTTACC179700.027.7387513
TACCTCT177850.027.65825316
CTAATGC177550.027.45330621
TTACCTC183450.027.02085715
GGACACC177150.026.8152629
ATGCTGG183500.026.43337224
CCTCTAA187550.026.28338218
Produced by FastQC (version 0.11.2)