FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23919287
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT549680.22980618109561543No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC495180.20702122099208056No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT365150.15265923269368353No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC288920.12078955363510625No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA269230.11255770291146219No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT264090.11040880942646827No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA262690.10982350769903801No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT261960.10951831465544938No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT248500.10389105661886995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG238650.037.5263255
TACTCTA145300.035.5192874
GCTGACT107850.034.935871
CTAGTTG151350.033.845548
TCTAGTT165200.031.9906067
TTGTACT158550.031.903391
CTCTAGT164150.031.6293496
GTACTCT164100.031.2399483
TGTACTC165900.030.9016462
GACTCTA141600.028.529354
ACCTCTA178600.028.1798417
TAGTTGT183850.028.0687839
GTTACCT180600.027.90573914
CTAGCAG141600.026.8457628
TGTTACC191950.026.65103513
CTGACTC143500.026.6136782
GGACACC183550.026.57616829
CTAATGC188100.026.55521821
TTACCTC197100.025.76226615
TACCTCT195800.025.7043916
Produced by FastQC (version 0.11.2)