FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25180764
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC348440.13837546787698737No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG297800.1182648786986765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG154600.027.558465
GCTGACT97800.027.0945531
GACTCTA127350.022.3217124
CTAGCAG117200.022.2511758
CTGACTC129150.020.6484262
TACTCTA80450.019.4683464
CTAGTTG81600.019.1921838
TCTAGTT98300.017.2657647
CTCTAGC172000.016.888156
CTCTAGT99600.016.6257136
CTATCGA156700.016.51014917
GGACACC85050.016.18415629
TCTATCG164750.015.80805816
TGACTCT183250.015.531353
TCTAGCA286400.015.3692227
GTTACCT99900.015.22776314
TAGCAGA178000.015.1545749
ACCTCTA101850.014.80059317
GACACCT94050.014.7451830
GTACTCT106250.014.7409743
Produced by FastQC (version 0.11.2)