FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20803634
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT588930.2830899639937907No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC523450.25161469385589075No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC314810.15132452339817168No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT290900.139831339082393No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT286570.1377499719520157No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT272580.13102518531137397No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA271640.13057334117683478No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA256210.12315636777689898No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT217420.10451058694841486No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC216320.10398183317395412No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTG211450.10164089600884153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT100100.037.95121
ACTCTAG178800.034.9614835
TACTCTA81350.029.6853924
CTGACTC132450.028.8077722
CTAGCAG134550.028.4321338
GACTCTA142800.028.3623494
CTAGTTG87550.027.3047818
TCTAGTT95850.025.9487387
CTCTAGT95550.025.7429926
GTACTCT100550.024.4286923
TGTACTC101600.023.9048162
TGACTCT172450.023.0057773
ACCTCTA101850.022.99706617
CTCTAGC172700.022.9124366
GTTACCT104900.022.62432314
TAGTTGT108000.022.358319
GACACCT103500.021.73134430
GGACACC105100.021.43385329
TTGTACT110600.021.3389591
TGTTACC112550.021.20940413
Produced by FastQC (version 0.11.2)