FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_nxtshoot2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_nxtshoot2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26462236
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT580030.21919160572825366No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC548980.2074579034061974No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC339960.12846986928844562No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT328190.12402202141950515No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT290850.10991134687182141No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT286860.10840353778116105No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA272940.10314321132953391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT116800.036.511711
ACTCTAG196750.030.0524255
CTGACTC159500.026.993882
GACTCTA169000.026.7004954
CTAGCAG163850.025.8951448
CTCTAGC215350.020.888976
TGACTCT213550.020.6943173
TAGCAGA216700.019.9139069
CTAGTTG77850.019.8951578
TACTCTA83750.018.5364254
CTATCGA229300.018.35363217
TCTATCG232750.018.12597516
TCTAGTT99950.017.1530237
TCTAGCA412450.017.0534787
GGACACC83100.017.02061829
GTGCGCA252700.016.86474668
GGTGCGC256700.016.64247167
TGCGCAT262600.016.28144869
ACCTCTA92050.016.00185617
GATCTAT265400.015.98706714
Produced by FastQC (version 0.11.2)