FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_damid_pdexdamgr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_damid_pdexdamgr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239614
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGA414320.9772587787473105No Hit
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGA361800.8533795765369205No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG214840.5067442460563626No Hit
CCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAG206600.4873085144072078No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG196440.46334406858737615No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC155810.36750987236102156No Hit
GGTCGCGGCCGAGGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCT138910.3276477528378763No Hit
GGTCGCGGCCGAGGATCATTTCATTTGGAGAGGACGTCGACCCTGCAGGCGGCCGCACTAGTATGAAGAAGAACA135060.31856673744355024No Hit
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGC122160.28813943910931517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG116210.27410514259081137RNA PCR Primer, Index 48 (100% over 43bp)
GGTCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCGAGGATCG109630.2585848617350542No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG107720.2540797346173496No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT93260.2199728560194395No Hit
GGTCGCGGCCGAGGATCAAATACAAAAAGAGGACAGCACGCCTAAAAGGGTCTTGAGACTTGTTAAATTCCTCAC83890.19787178738441755No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA80990.1910315420224577No Hit
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT79920.18850772735442425No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT78820.18591315152747398No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCGACGTCCTCTCCAAATGAAATGAACT76680.18086552219140703No Hit
CGGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCC73780.1740252768294472No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC73670.17376581924675216No Hit
GGTCGCGGCCGAGGATCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAG67030.15810401607316138No Hit
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCCTCGG66080.15586324604079524No Hit
GGTCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCG62260.14685299180538605No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT59520.14039013929098262No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT56950.13432826667710787No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC55620.13119118863179524No Hit
GGTCGCGGCCGAGGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTAT55480.1308609698901834No Hit
CCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGC52270.12328952588608302No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGCGTCCTTGGCTCGGATTTAGGCCAAC50020.1179824389673211No Hit
GGTCGCGGCCGAGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAA43150.10177813357536795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42650.10059878092675417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1704850.067.764281
GTCGCGG1722500.066.651442
TCGCGGC1776150.064.341443
CGCGGCC1810350.062.415194
CTAATAC30500.060.179491
GATCGGT37600.057.3412814
TAATACG32000.056.9205932
GCGGCCG1990300.056.92045
GGATCTT233400.056.90299613
CGGCCGA2002150.056.4577336
GGATCTA99650.056.3575813
AGGATCT502300.056.01173412
GATCTTA32650.055.99740214
GATCATT83850.055.9102714
GATCTAA23700.055.89308514
GGATCAT194500.055.7442413
GATCCAT129700.055.48167414
GATCATA27150.055.27065714
GGCCGAG2059550.055.077897
ATACGAC33200.054.550214
Produced by FastQC (version 0.11.2)