FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_damid_pdexbz1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_damid_pdexbz1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4128854
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGA277880.67301968052152No Hit
GCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGA246070.5959765106734217No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG227100.5500315583936851RNA PCR Primer, Index 47 (100% over 43bp)
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG220440.5339011745147685No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG197930.4793824145876798No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG190470.46131444705964414No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC161060.39008402815890314No Hit
CCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAG137450.33290109071427565No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC119030.2882882271933084No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT94600.22911926650833378No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT80830.19576860794787124No Hit
GGTCGCGGCCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGC77930.1887448672198145No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT76200.18455484257859445No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC75770.1835133913671929No Hit
GGTCGCGGCCGAGGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTAT67650.16384691732863405No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT66380.16077100328565747No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG65660.15902717800145028RNA PCR Primer, Index 47 (100% over 43bp)
GGTCGCGGCCGAGGATCTTTGGTTCGCGGGTCGTTCTGTTTTGGACAGGTATCGACAATGATCCTTCCGCAGGTT61470.14887908363918897No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG59210.14340540983042752No Hit
GGTCGCGGCCGAGGATCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAG58760.14231551902779804No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG53820.13035093999448757RNA PCR Primer, Index 47 (100% over 43bp)
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGCGTCCTTGGCTCGGATTTAGGCCAAC51750.1253374423023919No Hit
CGGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCCGAGGATCGCC45740.11078134513838465No Hit
CCGAGGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGCGATCCTCGGC44780.10845624475944174No Hit
GGTCGCGGCCGAGGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCTCGATCCT43530.10542877030769313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1717400.067.382451
GTCGCGG1742200.065.983922
TCGCGGC1806550.063.407453
TAATACG43400.062.0802352
CTAATAC43500.061.946521
CGCGGCC1836800.061.7960054
ACTATAG45650.057.5034413
GCGGCCG1980450.057.4628035
GGATCTA102050.057.22606713
CACTATA46400.056.5739712
GGATCTT255250.056.1236813
CGGCCGA2028650.056.015886
AGGATCT548150.055.93125512
ACCTAGG94350.055.83344347
CTATAGG46700.055.7680314
ATACGAC48100.055.65494
GATCTAT25800.055.2186214
GATCCAT160850.055.0932314
GGCCGAG2072800.054.965227
ATAGGGC47250.054.8275216
Produced by FastQC (version 0.11.2)