FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_damid_mdexdamgr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_damid_mdexdamgr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952198
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT51200.2622684789145363No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG49360.25284320545354516No Hit
GGTCGCGGCCGAGGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTT48600.24895015771965756No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG48090.24633771779296976No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA44550.2282043112430194No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG39600.20284827666046168No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT37530.19224484401684666No Hit
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA37390.19152770364481472No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC37060.18983730133931087No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC34090.17462368058977623No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG30770.1576172089101618No Hit
GGTCGCGGCCGAGGATCCACTGAGATTCAGCCCTTTGTCGCTAAGATTCGACCCTCCCCTAAATCACTCCAAAAA24540.12570446235474064No Hit
GGTCGCGGCCGAGGATCTTATATCGAACCACGATACTATGTTGACCAGAGAATGGTATCAAAGAGCAAAACTCCA24100.12345059261406885No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT22850.11704755357806944No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGCGTCCTTGGCTCGGATTTAGGCCAAC22520.11535715127256559No Hit
GGTCGCGGCCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA20540.1052147374395425No Hit
GGTCGCGGCCGAGGAACTATTGTTTTGTTAGGATTTTCCGAAGTGTCTTGTGAGACTCCTGCAGTGGCTTGCTGA20060.10275597044971874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG828800.067.6542741
GTCGCGG837900.066.843682
TCGCGGC862000.064.8434453
CGCGGCC884600.063.260914
CGGCCGA908550.061.537926
GGATCTA55850.061.3293813
GCGGCCG917650.061.099065
GGCCGAG916650.061.0503127
GGATCGG24200.059.7229413
AGGATCT261750.059.69873412
GGATCAT87650.059.660913
GATCCCT8850.059.63355314
GCCGAGG937850.059.615728
GGATCCA108550.059.5500713
AGGATCA225900.059.41534812
GATCCAT39250.059.40862714
GATCTAT17850.059.32567614
GATCATA21000.059.29668814
GGATCTT111100.059.26992813
GATCGGT9300.058.9734714
Produced by FastQC (version 0.11.2)