FastQCFastQC Report
Tue 13 Mar 2018
HMCN3BGX5_n01_damid_mdexbz1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCN3BGX5_n01_damid_mdexbz1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493219
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG67340.450972027545859RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT43700.29265633507208255No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG38200.2558231578890973No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG32270.21611029594453324No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG29490.19749279911386075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG29170.19534977789594157RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTCCCCTCGACGATTTCAAGTCCTGGTCGAGGAAT28290.18945646954666395No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC27370.18329528354514643No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG26650.17847348580482836No Hit
GGTCGCGGCCGAGGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTT25620.17157563625965114No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG25340.1697004926939719RNA PCR Primer, Index 45 (100% over 43bp)
GGTCGCGGCCGAGGATCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGA24050.16106143840923534No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC21810.14606028988380138No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA19190.1285143036620884No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGCGTCCTTGGCTCGGATTTAGGCCAAC17710.11860283052921239No Hit
GGTCGCGGCCGAGGATCCACTGAGATTCAGCCCTTTGTCGCTAAGATTCGACCCTCCCCTAAATCACTCCAAAAA17690.11846889170309244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17670.1183349528769725No Hit
GGTCGCGGCCGAGGATCGACGATTCCTCGGAACCGATTTTTTTCCCTCGACGATTTCAAGTCCTGGTGGAGGAAT17440.11679465637659313No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC15050.10078896665525953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG650700.068.162141
GTCGCGG664800.066.6964342
TCGCGGC700650.063.1065253
CGCGGCC730750.060.4599154
GATCTTA16150.058.31158414
GGATCTT92100.058.1669813
CGGCCGA762750.057.955086
GATCCAT36200.057.84220514
GCGGCCG769550.057.6222955
GATCATA18800.057.61512414
GGCCGAG769550.057.51477
AGGATCT221100.057.39918512
GCCGAGG780050.056.7727138
GGATCAT82750.056.44368713
GGATCCA92200.056.15836713
GGATCTG38350.056.12856713
GGATCTA46000.056.09297613
AGGATCA212100.056.0615812
GATCTAA14000.055.68588314
CCGAGGA787600.055.6591079
Produced by FastQC (version 0.11.2)