FastQCFastQC Report
Tue 30 May 2017
HMCH7BGX2_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCH7BGX2_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314914845
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28649350.9097491101126084No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT4846610.1539022398261346No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG4344320.1379522137166954No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC3422020.10866493130865267No Hit
GTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAA3253440.10331173813035077No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG3160740.10036808521999019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA289800.019.82322127
TATACCG2026500.019.6339995
GGACCAT1209800.019.5814556
ATACCGG1947900.019.3949786
GTTATAC1966250.019.3509463
CGGGTCC342300.018.3435845
TCAGGGG1918350.018.32098218
TTATACC2093250.018.112064
GGCTTCA1959100.017.78176514
GGTATTC1843400.017.66708424
ACCGGTG2092450.017.6183998
GGGGTAT1871550.017.52865422
TGGACCA1446900.017.4678765
GGGTCCA356900.017.463846
GGGTATT1878400.017.41411823
TTCAGGG2010150.017.32777817
TACCGGT2248300.017.297337
GGGGGTA2001800.017.24538421
CCGGTGG2071600.017.1678149
TGGCTTC2035250.017.01907213
Produced by FastQC (version 0.11.2)