FastQCFastQC Report
Tue 30 May 2017
HMCH7BGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMCH7BGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314914845
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAA4252300.013.8872151
TACACCT4408000.013.88308051
TTACCGT4531000.013.880151
TTCCAGA7577650.013.8724571
CCTCTCT3400250.013.8657764
CCACACT3974150.013.86140252
ACACCTT4414550.013.8606082
CACCTTC4407450.013.85093
TACTCCA3716350.013.8418533
CCTTCTT3499600.013.8384871
CACAGAA7322550.013.8332781
AACTACC4093500.013.8313426
TCGACCA3616850.013.8295842
TCAACCG3173200.013.8293751
TTCGACC3606600.013.8279971
TCAGAAC4112100.013.8269392
AATCCTT4130350.013.8265232
TCCTTAC4116450.013.8238014
CTACTCC3731950.013.818562
TTCCACC2482200.013.8159732
Produced by FastQC (version 0.11.2)