FastQCFastQC Report
Fri 16 Mar 2018
HM7TTBGX5_n02_bt22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n02_bt22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75415600
Sequences flagged as poor quality0
Sequence length150
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1649860.21876906104307334No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1532460.20320199003919615No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1501150.1990503291096272No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA1466710.19448363468566185No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1439820.19091805939354722No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1292550.17139026938723553No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1242630.16477094924657498No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1228150.1628509220903898No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG1197530.15879075416757277No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1164570.15442030561316225No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT1055100.1399047411941296No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1024390.13583263940086665No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA994070.13181225104620264No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA927070.12292814749203082No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC922860.12236990755228362No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT916220.12148945311049704No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA846220.11220755387479514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG843060.11178854242358345No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT836530.11092267382345297No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC832650.11040819140867406No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG791510.10495308662929155No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA790180.10477673054381323No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG778460.10322267541463569No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT765910.10155856348023486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA138100.062.0466881
GGTCTGG146800.060.1445163
TGTTACG167600.051.3482363
GGAGTCT267600.049.9948121
GAGTCTA271400.049.3232042
AGTCTAA276600.047.2038353
TTACGAC188400.046.4975245
GCTTGTA408300.046.0725677
CCTGGCC417300.044.3671885
CTTGTAA435150.043.3963938
AGCGCTT449050.042.153234
GTTACGA207150.042.0379524
GTCCAAT56350.041.273751
GTTGTGG91450.039.8411751
ACGACTT221550.039.4135677
TACCACT521600.039.2970167
TTGTAAT483300.039.103129
CGGATGT45150.038.7596743
GTGCGTC40350.038.2120329
TCTGGAG236700.037.5268365
Produced by FastQC (version 0.11.2)