FastQCFastQC Report
Fri 16 Mar 2018
HM7TTBGX5_n02_bt21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n02_bt21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61739269
Sequences flagged as poor quality0
Sequence length150
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1369020.22174217838568838No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA1277000.20683756394977723No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1156850.18737669213414238No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1149850.186242891861904No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1096440.17759199578472495No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1037130.16798546804951642No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1032980.16731328645954652No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG868880.14073376864892911No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT834340.13513927416276988No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA804850.13036273558729697No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA799500.1294961882363719No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA775810.12565908417218222No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG773040.1252104232073107No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC755170.12231599308375354No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG725020.11743255333975529No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA722190.11697417408683605No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT702760.11382706847403716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG688530.11152221449204396No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC679380.1100401755647609No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT649200.10515187667673877No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG626690.10150589894415499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA103950.054.8602451
GGAGTCT231900.051.2318731
GGTCTGG88550.051.1528473
GAGTCTA240000.049.5031172
AGTCTAA241900.047.1846923
CCTGGCC353350.046.0729375
GTCCAAT54900.045.510771
GCTTGTA221050.045.203297
TGTTACG131400.043.4595873
TTACGAC133400.043.2532965
CTTGTAA245050.040.98278
GTGCGTC53550.040.4951869
TGGCCCG407400.040.1587527
GCCTGTT26100.038.898751
AGCGCTT264700.037.847014
CGCGGAT48350.037.5284921
TCTAACG307350.037.218585
GTCAACT539100.036.98977
CGGATGT38250.036.900513
GTTACGA157200.036.697664
Produced by FastQC (version 0.11.2)